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Manuals and protocols

Guidelines for Biosafety and Biosecurity in Mosquito Rearing Facilities_Version 1.0

The present guidelines refer to a mosquito rearing facility which is suitable for work with uninfected mosquito vectors including: 1) mosquitoes that are already present in the local geographic region, and 2) exotic mosquitoes that upon escape would be inviable or become only temporarily established in areas not having active vector borne disease transmission.

Harmonized identification guideline of tefritids that might be considered of economic and quarantine importance in Latin America and the Caribbean

The correct identification of endemic fruit fly pests is fundamental for the design of effective integrated pest management programmes including those that apply the sterile insect technique (SIT). Also, for the enforcement of emergency action plans when
incursions of fruit fly quarantine species occur in an area. This manual is aimed at offering National Plant protection Organizations (NPPOs) with a harmonized guide that can support them in the continues
battle against fruit fly pests affecting food security and safety in the countries (manual only available in Spanish).

Guidance framework for testing the sterile insect technique as a vector control tool against Aedes-borne diseases

This document is intended to be a comprehensive guide for programme managers tasked with recommending a “go/no-go” decision on testing, full deployment and scale-up of the sterile insect technique (SIT) in regions of the world affected by diseases transmitted by Aedes mosquitoes. However, the authors hope that the material presented herein will be used more widely—by scientists, decision makers, review groups and others.

Guidelines for mark-release-recapture procedures of Aedes mosquitoess_v1.0.

The success of any Area-Wide Integrated Pest Management (AW-IPM) programme including a Sterile Insect Technique (SIT) component relies on the ability of the irradiated sterile males to survive, disperse and compete sexually with their wild counterparts to induce sterility in wild females. In a phased conditional approach for such a programme, it is considered essential to estimate these quality control parameters (survival, dispersal and competitiveness) in field conditions using mark-release-recapture (MRR) trials during the baseline data collection phase before shifting to a pilot trial. The protocols presented in this guideline are the results of lessons from collaborations with Member States preparing SIT pilot trials against Aedes species.

Guidelines for Blood Collection, Processing and Quality Control for Tsetse Rearing Insectaries, Version 2.0

This document is intended for use in laboratories and institutions that maintain colonies of tsetse flies. It describes the procedures for the collection of animal blood in the abattoir, decontamination through ionizing radiation, preservation and storage, quality control assurance and processing of the blood into diet for feeding tsetse flies.

Guidelines for mass rearing of Aedes mosquitoes_v1.0

The number and production capacity of mass-rearing insectaries for Aedes mosquitoes is increasing as the SIT technology is being validated in field pilot projects. These guidelines have been developed to provide a description of procedures required for Aedes aegypti and Aedes albopictus mass-rearing. It is a summary of necessary steps of larval and adult mass-rearing as used at the FAO/IAEA. This guide will be continuously updated considering the improvements brought to the mass-rearing of mosquitoes.

Product Quality Control for Sterile Mass-Reared and Released Tephritid Fruit Flies, Version 7.0.

FAO/IAEA/USDA. FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture. IAEA, Vienna, Austria (2019).

Standard Operating Procedures for Detection and Identification of Trypanosome Species in Tsetse Flies Version 1.0

The manual provides an easy-to-use work flow that combines several different PCR-based methods that can be used for trypanosome detection and identification in field-caught tsetse flies and/or host blood samples collected from livestock and wildlife animals.

Plan de Acción en Caso de Detección de Moscas de la Fruta No-Nativas Reguladas del Género Bactrocera spp En América Latina y El Caribe

Insect Pest Control Section of the Joint FAO/IAEA Division, International Atomic Energy Agency (IAEA) and Organismo Internacional Regional de Sanidad Agropecuaria (OIRSA). Vienna, Austria (May 2019).

Fruit sampling guidelines for area-wide fruit fly programmes

These guidelines are aimed at facilitating the transfer of harmonized sampling procedures to National Plant Protection Organizations and horticultural industry of FAO and IAEA Member States that want to apply fruit sampling procedures for fruit fly population survey in area-wide action programmes. Insect Pest Control Section of the Joint FAO/IAEA Division, International Atomic Energy Agency. Vienna, Austria, (June 2019).

Guidelines for Colonization of Aedes Mosquito Species - Version 1.0.

This document aims to provide a description of procedures required for the establishment of Aedes aegypti and Ae. albopictus colonies in your insectary or laboratory. It presents a summary of the necessary steps such as collecting material from the field, species identification, and adapting your wild colony to laboratory conditions and artificial rearing procedures. Insect Pest Control Section of the Joint FAO/IAEA Division, International Atomic Energy Agency. Vienna, Austria, (May 2018).

Update of the technical leaflet released in six languages on the discrimination between two invasive Bactrocera fruit fly pests

Bactrocera zonata (peach fruit fly) sometimes overlaps in terms of distribution with Bactrocera dorsalis (Oriental fruit fly). A technical leaflet to help to discriminate among these two invasive pest species, was updated and produced in Arabic, Chinese, English, French, Portuguese, and Spanish. The leaflet will be useful for countries and regions where these species are present or at risk of incursion helping to implement the recommended emergency response and is now available to regulatory agencies, surveillance and identification staff, growers of horticultural products and other stakeholders. It was prepared by Marc De Meyer (Royal Museum for Central Africa, Belgium) and Ian White (formerly research associate Natural History Museum, UK).

Mosquitoes SIT trifold

The document contains in a nutshell a description of SIT use against mosquitoes from mass rearing to sterilization and release. It also contains basic elements necessary for the cost-effective application of area-wide SIT technology against mosquitoes vectors of disease. Vienna, Austria (April 2018).

Manual para diferenciar moscas de Anastrepha ludens (Loew) silvestres

This manual includes standardised and updated procedures to determine the fertility or sterility of irradiated adults of wild and genetic sexing Anastrepha ludens strains. Insect Pest Control Section of the Joint FAO/IAEA Division, International Atomic Energy Agency. Vienna, Austria (January 2018).

Trapping Guideline for Area-Wide Fruit Fly Programmes

Insect Pest Control Section of the Joint FAO/IAEA Division, International Atomic Energy Agency. Vienna, Austria (January 2018)

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