Integrated Utilization of Cereal Mutant Varieties in Crop/Livestock Production Systems for Climate Smart Agriculture

Closed for proposals

Project Type

Coordinated Research Project

Project Code

D23030

CRP

1926

Approved Date

30 March 2012

Status

Closed

Start Date

17 September 2012

Expected End Date

17 September 2017

Completed Date

16 May 2018

Description

There are three basic components to the work plan: 1) Develop cropping systems for cereal mutant varieties for improved food and feed production; 2) Improve farmer productivity; and 3) Evaluation.In compliance with recommendation No. 34: “Develop a CRP on mutant varieties for fodder” from the AGE Week (September 2011), an inter-disciplinary concept was drafted on the utilization of crop mutants for improved fodder quality. This concept note formed the basis of a Consultants Meeting held at the IAEA in Vienna (12-16th December 2011). Consultants were invited to develop the concept note into a proposal for a CRP; in addition 2 observers from the FAO (Rome) also attended along with relevant FAO/IAEA staff in Vienna. During the course of discussions it was decided that the new CRP should focus on cereals. The CRP proposes to develop agronomic practices that maximise yield and nutritional value of cereals as dual purpose food and feed crops using a range of contemporary mutant varieties. Cereals are grasses with edible starchy grains and include many staple crops, e.g. barley, maize, rice, sorghum and wheat. They are harvested for their grain for human consumption, but may also be harvested throughout their life cycle as fodder for animals. Cereals are adapted to a wide range of growing conditions, barley, for example, is a useful food and feed crop in cool, dry conditions whereas maize, for example, fits into cropping systems with a warm wet season. One of the first steps is to select existing mutant varieties in a range of countries. The type of mutant variety used will vary depending on the participating country. These will then be used in developing soil and water management practices that maximise biomass production. For fodder production, this often occurs before flowering, i.e. much earlier than standard harvests at grain maturity The CRP will establish the base line for agronomic practice for biomass production and feed value. It is important to note that in developed countries cereals are commonly separated into food and feed varieties, but this is a new concept for developing countries. It was made clear by the consultants that farmers in developing countries need cereal crops that can be exploited for both food and feed. It was also stressed that farmers should have the ability to vary the use of the crop depending on circumstances; such flexibility is important, ideally the crop may be harvested at any time as fodder (animal consumption). It may be sown and harvested early for rapid green fodder production or grown to maturity for grain yield (human consumption). The CRP aims to develop soil and water management systems that maximise yields of dual purpose (food and feed) mutant cereal varieties. Progress will be measured in terms of performance in the field (yield), and by animal and crop nutrition studies. The project involves three FAO/IAEA disciplines: 1) plant breeding and genetics, 2) soil and water management, and 3) animal nutrition. It is recommended that participating Member States (MSs) have in place (or put in place) integrated programmes involving all three disciplines. This is not expected to be a problem and the consultants were able to identify at least 6 countries where this was the case for barley alone (Brazil, China, India, Kenya, Peru and Uruguay). It is anticipated that more countries will apply than can be handled in a well-focused CRP programme. It was also acknowledged that there are many mutants of interest in developing cereal fodder crops, but that many of these are present in genetic stocks and not current varieties and would require a large pre-breeding effort outside the scope of this CRP. However, to accommodate demand for mutant stocks, it was agreed, where appropriate, that germplasm containing a range of target mutant traits (awnless, hooded, low lignin, etc) would be made available to all interested countries for use in other national programmes.

Objectives

To develop cropping systems for mutant cereal varieties for food and feed. To demonstrate the utility of dual purpose cereal crops for enhanced food security in small holder crop-livestock production systems in developing countries.

Specific objectives

1. To develop crop management systems for cereal mutant varieties and advanced lines carrying mutant genes with respect to improved yield and quality.

2. To evaluate mutant cereal varieties for agronomic performance and feed quality.

3. To multiply seed of superior lines for fodder production trials.

4. To evaluate the nutritive value of new mutant lines in animal production systems.

5. To determine biomass, harvest index and nitrogen-use efficiency of mutant varieties and advanced lines.

6. To validate and publish protocols and guidelines for speeding up the establishment of useful mutants in desirable genetic backgrounds.

7. To perform pilot tests of superior mutant varieties/lines on-farm through participatory farmer approaches.

Impact

Barley is a major cereal crop for feed consumed by a wide range of animals including cows, pigs and poultry as green forage, hay, silage or grain. Multiple mutations exist in barley that can be exploited to develop specific forage barley varieties. Barley ‘orange lemma’ (rob1) mutants show similar reduced lignin content to mutants in maize. Molecular marker for low-lignin trait (rob1) (recessive) useful as animal feed, Barley Orange Lemma marker was developed to improve livestock feed; it is perfect’ functional marker and saves significant time and resources (labor, space) vs phenotyping. Technology is available for Member States also it has been transferred to several Member States such as Austria, Kuwait and Tunisia.

Relevance

Introductory guide and laboratory Protocols developed and shared with Member States on “Low-Cost Genotyping Protocol and Kit for Marker-Assisted Selection of Orange Lemma (rob1.a), a Feed Quality Trait in Barley (Hordeum vulgare L.) “

CRP Publications

Type

abstract, poster

Year

2013

Publication URL

http://viscea.org/

Description

Taassob-Shirazi F., Forster B.P., Huang B., Grausgruber H., Franckowiak J. (2013): Rapid introgression of mutant traits for fodder quality in barley. [Poster] [International Conference Plant Genetics and Breeding Technologies, Vienna, 18-20 February] In: VISCEA (Vienna International Science Conferences & Events Association), Proceedings of the Genetic Transformation Technologies (plants and animals), Plant Genetics and Breeding Technologies, Plant Diseases and Resistance Mechanisms Conferences, 14-22 February 2013, Vienna, Austria, Medimond s.r.l. - Monduzzi Editore International Proceedings Division, Pianoro, Italy, pp. 53-56. ISBN 978-88-7587-682-1

Country/Organization

Austria

Type

Symposium Poster

Year

2018

Publication URL

https://conferences.iaea.org/event/145/contributions/12475/contribution.pdf

Description

Development of a Functional Marker for Marker-Assisted Selection of ‘Orange Lemma’ Mutants to Improve Feed Quality in Barley

Country/Organization

Joint FAO/IAEA Centre

Type

Poster

Year

2018

Publication URL

https://www.iaea.org/sites/default/files/18/08/cn-263-abstracts.pdf

Description

Evaluation of hooded (Kap1), awnless (Lks1) and orange lemma (rob1) muants of barley (Hordeum vulgare L.) for their use as forage crop

Country/Organization

Joint FAO/IAEA Centre

Type

Book of abstract

Year

2013

Publication URL

http://viscea.org/

Description

Bernhard Hofinger, Owen Huynh, Joanna Jankowicz-Cieslak, BiguangHuang, Isabelle Henry, Luca Comai, JochenKumlehn, Brian Forster, andBradley Till *. Choose your throughput: recovery of induced and naturalnucleotide variation in plants. VIPCA conference, Vienna Feb. 2013. Book ofabstracts.

Country/Organization

China

Type

Book of Abstracts, Conference & Workshop proceedings, paper, abstract

Year

2014

Description

Grausgruber H, Atzgersdorfer K, Taassob-Shirazi F, Bohmdorfer S, ForsterBP (2014) Cereal quality - time for a paradigm shift? In: Lohwasser U.,Borner A. (eds.), EUCARPIA Cereals SectionllTMI Joint Conference,Cereals for Food, Feed and Fuel - Challenge for Global Improvement, 29June - 4 July 2014, Wernigerode, Germany, Book of Abstracts, p. 99.

Country/Organization

Austria

Type

Book of Abstracts, Conference & Workshop proceedings, paper, abstract

Year

2014

Description

Grausgruber H, Atzgersdorfer K, Taassob-Shirazi F, Böhmdorfer S, Forster BP (2014) Cereal quality - time for a paradigm shift? In: Lohwasser U., Börner A. (eds.), EUCARPIA Cereals Section/ITMI Joint Conference, Cereals for Food, Feed and Fuel - Challenge for Global Improvement, 29 June - 4 July 2014, Wernigerode, Germany, Book of Abstracts, p. 99.

Country/Organization

Austria

Type

book of abstracts, poster

Year

2013

Publication URL

https://pag.confex.com/pag/xxi/webprogram/Paper7462.html

Description

Taassob-Shirazi F., Huang B., Grausgruber H., Franckowiak J., Forster B. (2013): Introgression of mutant alleles in barley. [Plant & Animal Genome XXI, San Diega, CA, 12-16 January]

Country/Organization

Austria

Type

abstract, poster

Year

2013

Publication URL

http://viscea.org/

Description

Taassob-Shirazi F., Forster B.P., Huang B., Grausgruber H. , Franckowiak J.(2013): Rapid introgression of mutant traits for fodder quality in barley.[Poster] [International Conference Plant Genetics and BreedingTechnologies, Vienna, 18-20 February] In: VISCEA (Vienna InternationalScience Conferences & Events Association), Proceedings of the GeneticTransformation Technologies (plants and animals), Plant Genetics andBreeding Technologies, Plant Diseases and Resistance MechanismsConferences, 14-22 February 2013, Vienna, Austria, Medimond s.r.1. -Monduzzi Editore International Proceedings Division, Pianoro, Italy, pp. 53-56. ISBN 978-88-7587-682-1

Country/Organization

Austria

Type

book of abstracts

Year

2013

Description

Bernhard Hofinger, Owen Huynh, Joanna Jankowicz-Cieslak, BiguangHuang, Isabelle Henry, Luca Comai, JochenKumlehn, Brian Forster, andBradley Till *. Choose your throughput: recovery of induced and naturalnucleotide variation in plants. VIPCA conference, Vienna Feb. 2013. Book ofabstracts.

Country/Organization

China

Type

Book of Abstracts, Conference & Workshop proceedings, paper, abstract

Year

2014

Description

Grausgruber H, Atzgersdorfer K, Taassob-Shirazi F, Bohmdorfer S, ForsterBP (2014) Cereal quality - time for a paradigm shift? In: Lohwasser U.,Borner A. (eds.), EUCARPIA Cereals SectionllTMI Joint Conference,Cereals for Food, Feed and Fuel - Challenge for Global Improvement, 29June - 4 July 2014, Wernigerode, Germany, Book of Abstracts, p. 99.

Country/Organization

Austria

Type

research article

Year

2014

Publication URL

http://dx.doi.org/10.1155/2014/190531

Description

Genetic Variability and Selection Criteria in Rice Mutant Lines as Revealed by Quantitative Traits, Yusuff Oladosu, M. Y. Rafii, Norhani Abdullah, Mohammad Abdul Malek, H. A. Rahim, Ghazali Hussin, Mohammad Abdul Latif, and Isiaka Kareem

Country/Organization

Malaysia

Type

book of abstracts, poster

Year

2013

Description

Taassob-Shirazi F., Huang B., Grausgruber H., Franckowiak J., Forster B.(2013): Introgression of mutant alleles in barley. [Plant & Animal GenomeXXI , San Diega, CA, 12-16 January]

Country/Organization

Austria

Type

Protocol

Year

2020

Publication URL

https://www.iaea.org/sites/default/files/21/06/nafa-pbg-manual-diagnostic-marker…

Description

A Low-Cost Genotyping Protocol and Kit for Marker-Assisted Selection of Orange Lemma (rob1.a), a Feed Quality Trait in Barley (Hordeum vulgare L.)

Country/Organization

Joint FAO/IAEA Center, PBG

Type

abstract, poster

Year

2013

Publication URL

http://viscea.org/

Description

Taassob-Shirazi F., Forster B.P., Huang B., Grausgruber H. , Franckowiak J. (2013): Rapid introgression of mutant traits for fodder quality in barley. [Poster] [International Conference Plant Genetics and BreedingTechnologies, Vienna, 18-20 February] In: VISCEA (Vienna InternationalScience Conferences & Events Association), Proceedings of the GeneticTransformation Technologies (plants and animals), Plant Genetics andBreeding Technologies, Plant Diseases and Resistance MechanismsConferences, 14-22 February 2013, Vienna, Austria, Medimond s.r.1. -Monduzzi Editore International Proceedings Division, Pianoro, Italy, pp. 53-56. ISBN 978-88-7587-682-1

Country/Organization

Austria

Type

Research Article

Year

2014

Publication URL

https://www.hindawi.com/journals/tswj/2014/190531/

Description

Genetic Variability and Selection Criteria in Rice Mutant Lines as Revealedby Quantitative Traits, Yusuff Oladosu, M. Y. Rafii, Norhani Abdullah,Mohammad Abdul Malek, H. A. Rahim, Ghazali Hussin, Mohammad AbdulLatif, and Isiaka Kareem

Country/Organization

Malaysia

Type

Protocol

Year

2020

Publication URL

https://www.iaea.org/sites/default/files/21/06/nafa-pbg-manual-diagnostic-marker…

Description

A Low-Cost Genotyping Protocol and Kit for Marker-Assisted Selection of Orange Lemma (rob1.a), a Feed Quality Trait in Barley (Hordeum vulgare L.)

Country/Organization

Joint FAO/IAEA Centre

Type

book of abstracts

Year

2013

Description

Bernhard Hofinger, Owen Huynh, Joanna Jankowicz-Cieslak, Biguang Huang, Isabelle Henry, Luca Comai, JochenKumlehn, Brian Forster, and Bradley Till*. Choose your throughput: recovery of induced and natural nucleotide variation in plants. VIPCA conference, Vienna Feb. 2013. Book of abstracts.

Country/Organization

China

Type

Research Article

Year

2015

Publication URL

http://dx.doLorg/10.1155/2015/290861

Description

Genetic Variation, Heritability, and Diversity Analysis of Upland Rice (Oryzasativa L.) Genotypes Based on Quantitative Traits, Mst. Tuhina-Khatun,Mohamed M. Hanafi, Mohd Rafii Yusop, M. Y. Wong, Faezah M. Salleh, andJannatul Ferdous

Country/Organization

Malaysia

Type

research article

Year

2014

Publication URL

http://dx.doi.org/10.1155/2014/190531

Description

Genetic Variability and Selection Criteria in Rice Mutant Lines as Revealedby Quantitative Traits, Yusuff Oladosu, M. Y. Rafii, Norhani Abdullah,Mohammad Abdul Malek, H. A. Rahim, Ghazali Hussin, Mohammad AbdulLatif, and Isiaka Kareem

Country/Organization

Malaysia

Type

book of abstracts, poster

Year

2013Taassob-Shirazi F., Huang B., Grausgruber H., Franckowiak J., Forster B. (2013): Introgression of mutant alleles in barley. [Plant & Animal GenomeXXI , San Diega, CA, 12-16 January]

Country/Organization

Austria

Type

Research Article

Year

2

Publication URL

https://www.hindawi.com/journals/bmri/2015/290861/

Description

Genetic Variation, Heritability, and Diversity Analysis of Upland Rice (Oryzasativa L.) Genotypes Based on Quantitative Traits, Mst. Tuhina- Khatun,Mohamed M. Hanafi, Mohd Rafii Yusop, M. Y. Wong, Faezah M. Salleh, andJannatul Ferdous

Country/Organization

Malaysia

Type

Research Article

Year

2015

Publication URL

http://dx.doi.org/10.1155/2015/290861

Description

Genetic Variation, Heritability, and Diversity Analysis of Upland Rice (Oryza sativa L.) Genotypes Based on Quantitative Traits, Mst. Tuhina-Khatun, Mohamed M. Hanafi, Mohd Rafii Yusop, M. Y. Wong, Faezah M. Salleh, and Jannatul Ferdous

Country/Organization

Malaysia

Stay in touch

Newsletter