Use of Stable Isotopes to Trace Bird Migrations and Molecular Nuclear Techniques to Investigate the Epidemiology and Ecology of the Highly Pathogenic Avian Influenza

Closed for proposals

Project Type

Coordinated Research Project

Project Code

D32030

CRP

1890

Approved Date

14 December 2011

Status

3 - Active - Ongoing

Start Date

9 July 2012

Expected End Date

9 July 2017

Completed Date

25 January 2019

Participating Countries

Canada
Germany
Nepal
Republic of Korea
Russian Federation
United Kingdom of Great Britain and Northern Ireland

Description

Integrating information on wild waterfowl migration through stable isotope analysis and detecting avian influenza viruses in faecal and environmental samples will contribute to understanding the epidemiology and ecology of the long range transmission of avian influenza viruses, using non invasive methods.

Objectives

1) Evaluate the potential of precipitation deuterium isoscapes based on the IAEA GNIP program for determining origin of waterfowl in Africa/Asia by calibrating this isoscape with feathers (and potentially claws) grown at known origins.2) Utilize species-specific telemetry and other marking data to identify waterfowl flyways including stopover, breeding, non-breeding and moult locations in Africa/Asia and use these data to inform isotopic sampling strategies.3) Evaluate tissues from waterfowl (feather, blood and claws) samples previously collected and identify key samples for isotope analysis to support objectives 1 and 2.4) Evaluate the implementation of non-invasive methods for AI surveillance in WWF, based on simultaneous DNA barcoding and detection of the AIV in faecal samples.5) Evaluate the level of eventual contamination and the risk of AIV transmission in WWF via natural water reservoirs.

Specific objectives

Determine the potential use of stable isotope analysis in tracing migratory pathways of wild water fowl.

Develop validate SOPs for detection and quantitative evaluation of the AIV in natural water reservoirs.

Develop validated SOP for DNA barcoding for differentiation of WWF species using faecal samples.

Develop validated SOP for DNA barcoding for differentiation of WWF species using fecal samples.

Develop validated SOPs for detection and typing of the AIV in WWF using faecal samples.

Develop validated SOPs for detection and typing of the AIV in WWF using fecal samples.

Improve the existing isoscapes for dD and other essential isotopes, especially in the regions of Asia and Africa.

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