Application of Nuclear and Genomic Tools to Enable the Selection of Animals with Enhanced Productivity Traits

Closed for proposals

Project Type

Coordinated Research Project

Project Code

D31028

CRP

2105

Approved Date

9 December 2015

Status

Closed

Start Date

31 May 2016

Expected End Date

30 November 2021

Completed Date

27 April 2024

Participating Countries

Argentina
Austria
Bangladesh
India
Italy
Kenya
Peru
Serbia
South Africa
Sri Lanka
Tunisia
United Kingdom of Great Britain and Northern Ireland

Description

The World will be facing the challenge of manifolds increase in the production of food from animal origin to address the high demand that is expected to arise from population growth, income increases and urbanization. Breeding for robust animals with production systems optimised for exponential increase in productivity while retaining their adaptability to harsh environment and tolerance to tropical diseases could remain only option for the intensification of livestock productions with as minimum as possible environmental impacts. Crossbreeding zebu cattle with temperate taurine cattle has been tried in many countries for a rapid increase in the productivity but results were not always satisfactory. Lack of animal identification and not having a system in place for recording and analysing performance data to make appropriate breeding decision were identified to be main constraints that limited the enhancement of animal productivity by breeding, especially in developing countries. This project aims the application of nuclear and nuclear-derived molecular techniques to addresses two major issues prevailing in developing countries and are directly associated with food security and livelihood improvement. Firstly, it will generate genomic data of performance recorded animals, which will enable breeders and farmers relate production traits with parentage and genetic admixture of animals leading to identification and selection of superior (both genotypically and phenotypically) sires for breeding by using artificial insemination. Secondly, 60CO will be applied to develop a radiation hybrid panel of camel and use that for whole genome sequencing and identification of breeding markers that nobody has done yet for camel.  As outputs the project is expected to leave bind an animal identification system in place and 1000 phenotype recorded animals per breed/population from each country, develop a gene bank of phenotype recorded animals in participating countries (potentially available for future genotyping), validate genetic tool(s) for testing parentage, relationship and admixture level, develop whole genome radiation hybrid panels for camel, develop a set of performance data for different genetic groups in different production systems and deliver standard operating procedures (SOPs), protocols and guidelines for continued animal genetic research and application of results in animal breeding. The project will be running five years and will involve 10 Research Contract (RC) holders from developing countries, three Technical Contract (TC) holders and four Research Agreement (RA) holders from laboratories engaged in high level animal genetics and breeding research and field application. The project budget is estimated to €615,000.-.

Objectives

To enable MS, especially developing countries to use genomic tools for enhancing the efficiency and effectiveness of genetic improvement of livestock.

Specific objectives

Strengthen research capacity of animal scientists in developing countries.

To strengthen animal identification, pedigree and performance recording system in large ruminants under AI programmes and camels

To develop and characterize radiation hybrid panels for mapping camel genome

To implement parentage testing, performance recording and admixture analysis of females bred by AI sires

Networking among animal scientists from developed and developing countries.

To characterize sires under AI programmes for whole genome variations, as a basis for future selection

Impact

The CRP has a significant positive impact in member countries, particularly in improving the efficiency of dairy cattle breeding programs for increased milk productivity. The genomic tools developed under the CRP (e.g., multi-species camelid DNA chip) are already being used in more than 20 countries. The DNA bank of performance recorded animals established in member countries under the CRP will form a basis for creating reference populations that can be utilized to develop genomic prediction machinery and implement genomic selection in future.

Relevance

The CRP is highly relevant for livestock development in member states, especially targeting production efficiency gains.

CRP Publications

Type

Peer Reviewed Research Paper

Year

2022

Publication URL

https://road.iaea.org/livelink/llisapi.dll?func=ll&objId=102242104&objAction=Ope…

Description

Diversity of the BoLA-DRB3 gene in cattle breeds from tropical and subtropical regions of Argentina

Country/Organization

Argentina

Type

Peer Reviewed Research Paper

Year

2020

Publication URL

https://road.iaea.org/livelink/llisapi.dll?func=ll&objId=102216034&objAction=Ope…

Description

Protein Synthesis by Day 16 Bovine Conceptuses during the Time of Maternal Recognition of Pregnancy

Country/Organization

United Kingdom

Type

Peer Reviewed Research Paper

Year

2017

Publication URL

https://road.iaea.org/livelink/llisapi.dll?func=ll&objId=102173110&objAction=Ope…

Description

BOLA-DRB3 gene polymorphisms influence bovine leukaemia virus infection levels in Holstein and Holstein 3 Jersey crossbreed dairy cattle

Country/Organization

Argentina

Type

Peer Reviewed Research Paper

Year

2020

Publication URL

https://road.iaea.org/livelink/llisapi.dll?func=ll&objId=102173109&objAction=Ope…

Description

BoLA-DRB3 exon2 polymorphisms among tuberculous cattle: Nucleotide and functional variability and their association with bovine tuberculosis pathology

Country/Organization

Argentina

Type

Peer Reviewed Research Paper

Year

2018

Publication URL

https://road.iaea.org/livelink/llisapi.dll?func=ll&objId=102245591&objAction=Ope…

Description

Construction of two whole genome radiation hybrid panels for dromedary (Camelus dromedarius): 5000RAD and 15000RAD

Country/Organization

Multiple countries

Type

Peer Reviewed Research Paper

Year

2020

Publication URL

https://road.iaea.org/livelink/llisapi.dll?func=ll&objId=102173111&objAction=Ope…

Description

Short Tandem Repeat (STR) based assessment of genetic diversity of Alambadi– A draught cattle breed of Tamil Nadu

Country/Organization

India

Type

Peer Reviewed Research Paper

Year

2021

Publication URL

https://road.iaea.org/livelink/llisapi.dll?func=ll&objId=102245590&objAction=Ope…

Description

Phylogenetic relationships of Argentinean Creole with other Latin American Creole Cattle as revealed by a medium density Single Nucleotide Polymorphism microarray

Country/Organization

Argentina

Type

Peer Reviewed Research Paper

Year

2017

Publication URL

https://road.iaea.org/livelink/llisapi.dll?func=ll&objId=102245243&objAction=Ope…

Description

Prevalence and its influencing risk factors of subclinical mastitis in crossbred Friesian cows

Country/Organization

Bangladesh

Type

Peer Reviewed Research Paper

Year

2020

Publication URL

https://road.iaea.org/livelink/llisapi.dll?func=ll&objId=102222565&objAction=Ope…

Description

Identification of reliable reference genes for expression studies in maternal reproductive tissues and foetal tissues of pregnant cows

Country/Organization

China

Type

Peer Reviewed Research Paper

Year

2020

Publication URL

https://road.iaea.org/livelink/llisapi.dll?func=ll&objId=102242105&objAction=Ope…

Description

BETA-CASEIN GENE POLYMORPHISM IN SERBIAN HOLSTEIN-FRIESIAN COWS AND ITS RELATIONSHIP WITH MILK PRODUCTION TRAITS

Country/Organization

Serbia

Type

Book Chapter

Year

2023

Publication URL

https://road.iaea.org/livelink/llisapi.dll?func=ll&objId=102190304&objAction=Ope…

Description

Applying genomics to identify optimal crossbred cattle genotypes for sustainable smallholder dairy production in Bangladesh.

Country/Organization

Bangladesh

Type

Peer Reviewed Research Paper

Year

2018

Publication URL

https://road.iaea.org/livelink/llisapi.dll?func=ll&objId=102241819&objAction=Ope…

Description

Single nucleotide polymorphisms in candidate genes associated with milk yield in Argentinean Holstein and Holstein x Jersey cows

Country/Organization

Argentina

Type

Peer Reviewed Research Paper

Year

2018

Publication URL

https://road.iaea.org/livelink/llisapi.dll?func=ll&objId=102174453&objAction=Ope…

Description

Genome-wide scan for commons SNPs affecting bovine leukemia virus infection level in dairy cattle

Country/Organization

Argentina

Type

Peer Reviewed Research Paper

Year

2018

Publication URL

https://road.iaea.org/livelink/llisapi.dll?func=ll&objId=102216033&objAction=Ope…

Description

Pregnancy rate and associated factors in dairy cows of Bangladesh

Country/Organization

Bangladesh

Type

Peer Reviewed Research Paper

Year

2018

Publication URL

https://road.iaea.org/livelink/llisapi.dll?func=ll&objId=102174454&objAction=Ope…

Description

Bovine foetal sex determination—Different DNA extraction and amplification approaches for efficient livestock production

Country/Organization

Serbia

Type

Peer Reviewed Research Paper

Year

2021

Publication URL

https://road.iaea.org/livelink/llisapi.dll?func=ll&objId=102241820&objAction=Ope…

Description

Legacy of draught cattle breeds of South India: Insights into population structure, genetic admixture and maternal origin

Country/Organization

India

Type

Peer Reviewed Research Paper

Year

2020

Publication URL

https://road.iaea.org/livelink/llisapi.dll?func=ll&objId=102245244&objAction=Ope…

Description

THE EFFECTS OF ANTIOXIDANTS PROVIDED WITH FEED ON CERTAIN QUALITY PARAMETERS OF BULL SEMEN UNDER HEAT STRESS CONDITIONS

Country/Organization

Serbia

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