Identification and Pyramiding of Mutated Genes: Novel Approaches for Improving Crop Tolerance to Salinity and Drought

Closed for proposals

Project Type

Coordinated Research Project

Project Code

D23026

CRP

1360

Approved Date

29 April 2004

Status

Closed

Start Date

1 December 2004

Expected End Date

30 November 2010

Completed Date

30 November 2010

Objectives

To identify and develop germplasm of cereals and grain legumes with superior resource use efficiency and adapted to stress environments, in order to increase its future application to breeding programmes in member states.

Specific objectives

A. To generate genetic variability, and to use existing mutated and naturally tolerant germplasm of crop plant genetic resources to identify genes controlling various traits contributing to tolerance to drought and salinity in defined environments and so gain a better understanding of the physiological and molecular basis of plant tolerance to drought and salinity.

B. To utilize refined nuclear and molecular techniques for screening large germplasm populations for improved yield and water use efficiency under saline and drought conditions.

C. To pyramid (create a platform for pyramiding) identified genes/characters and genotypes for effective improvement of stress tolerance in locally important cereals and grain legumes which contribute to food security using marker-assisted and other biotechnologies as well as farmer-participatory selection.

D. To foster relationships, transfer knowledge, technology, and genetic and molecular resources between all participating research groups for their mutual benefit.

Impact

The CRP allowed very good interactions and collaborations between all the participants, thus impacting directly the research output of these institutions and also enriching the scientist with new methodologies for implementation in their breeding and research programmes. A few advanced lines were reported, which should in the next future be introduced in the national crop improvement programmes and also the screening protocols designed under the CRP will surely be of great assistance to other scientists and breeders.
Specific achievements
• One Gene identified: oxoglutarate-dependent dioxygenase (At3G13610.1) part of oxidative stress network in Arabidopsis thaliana.
• Generation of durum wheat TILLING population.
• Construction and primary characterization of three mutant populations (M5) of soybean
• Identification of two rice mutants with significant tolerance to salinity.
• EcoTILLING in durum wheat initiated
• Confirmed 7 gain of function (GOF) rice mutants with enhanced drought tolerance/aerobic adaptation.

Relevance

The CRP was of outmost relevance, as it was the first to deal with the pyramiding of mutated genes, which should become a routine procedure when working on the improvement relating to multigenic traits.

CRP Publications

Type

Scientific articles

Year

2008-2009

Description

• Moustafa, R.A.K (2008). Utilization of gamma irradiation for improving salinity tolerance of two exotic hull-less barley varieties. 9th Conference of Nuclear Sciences and App ications. 11-14, February, 2008. Cairo-Egypt.27-35.• Moustafa,R.A.K (2008). Development of salt tolerance high yielding barley lines via crossing between a mutant induced by EMS and a local cultivar. International Symposium on Induced Mutations in Plants. 12-15 August2008. Vienna, Austria.• Moustafa,R.A.K and H.G.M.,Ali (2009). Evaluation of some chemical and technological properties of induced erect chickpea mutant lines developed under drought stressed conditions. Journal of Radiation Research and Applied science. (In press).

Country/Organization

Tunisia

Type

Scientific articles

Year

2008-2009

Description

• Kushwaha HR, Singh AK, Sopory SK, Singla-Pareek SL and Pareek A (2009) Genome wide expression analysis of CBS domain containing proteins in Arabidopsis thaliana (L.) Heynh and Oryza sativa L. reveals their developmental and stress regulation. BMC Genomics 10: 200-210• Karan R, Singla-Pareek SL and Pareek A (2009) Histidine kinase and response regulator genes as they relate to salinity tolerance in rice. Functional and Integrative Genomics 9:411-7.• Kumari S, Singh P, Singla-Pareek SL and Pareek A (2009) Heterologous expression of a salinity and developmentally regulated rice cyclophilin gene (OsCyp2) in E. coli and S. cerevisiae confers tolerance towards multiple abiotic stresses. Molecular Biotechnology 42:195-204.• Kumar G, Purty RS, Singla-Pareek SL and Pareek A (2009) Maintenance of stress related transcripts in tolerant cultivar at a level higher then sensitive one appears to be a conserved salinity response among plants. Plant Signalling and Behaviour 4:1-4.• Kumar G, Purty RS, Sharma MP, Singla-Pareek SL and Pareek A (2009) Physiological responses among Brassica species under salinity stress show strong correlation with transcript abundance for SOS pathway related genes. Joumal of Plant Physiology 166: 507-520.• Kumari S, Panjabi nee Sabharwal V, Kushwaha H R, Sopory SK, Singla-Pareek SL and ' Pareek A (2009) Transchptome map for seedling stage specific salinity stress response indicates a specific set of genes as candidate for saline tolerance in Oryza sativa L. Functional and Integrative Genomics 9:109-123.• Kumar G, Purty RS, Sharma MP, Singla-Pareek SL and Pareek A (2009). Physiological responses among Brassica species under salinity stress show strong correlation with transcript abundance for SOS pathway related genes. Journal of Plant Physiology 166: 507-520.• Purty RS, Kumar G, Singla-Pareek SL and Pareek A (2008) Invited contribution Towards Salinity Tolerance in Brassica: an Overview, Physiol. Mol. Biol. Plants: 14:15-22• Singh AK, Ansari MW, Pareek A and Singla-Pareek SL (2008) Invited contribution Raising Salinity Tolerant Rice: Recent Progress and Future Perspectives, Physiol. Mol. Biol. Plants. 14: 23-32• Singla-Pareek SL, Yadav SK, Pareek A, Reddy MK and Sopory SK (2008). Enhancing salt tolerance in a crop plant by overexpression of glyoxalase II. Transgenic Research. 17:171-180.

Country/Organization

India

Type

Scientific articles

Year

2009

Description

• Bovina R, Tálame V, Salvi S., Sanguineti MC, Tuberosa R 2009. Discovery of Chemically Induced Mutations by TILLING. In: Q. Shu, (Editor) "/Molecular Techniques for Mutation Detection and Their Applications/", Springer, Dordrecht, (in press).• Bovina R., Tálame V., Salvi S., Sanguineti M. C, Szarejko I., Fincher G, Tuberosa R. TILLMore: a forward and a reverse genetics resource for the identification of root morphology-related mutants Plant GEM 07/10 October 2009, Lisbon, Portugal.

Country/Organization

Italie

Type

Book

Year

2012

Description

Guidelines on Crop Improvement for Salinity Tolerance in Asia

Country/Organization

FAO/IAEA Joint Division

Type

Scientific articles

Year

2006-2009

Description

• Gechev T, Ferwerda M, Mehterov N, Qureshi M, Denev I, Laloi C, Toneva V, Radeva V, Minkov I, Hille J (2009) Mutational analysis to dissect oxidative and abiotic stress in Arabidopsis thaliana. Proc. Int. Simp. Ind. Mut. Plants, FAO, 147-150• Gechev T, Ferwerda M, Mehterov N, Laloi C, Qureshi M, Hille J (2008) Arabidopsis AALtoxin- resistant mutant atrl shows enhanced tolerance to programmed cell death induced by reactive oxygen species. Biochem. Biophys. Res. Commun. 375: 639-644• Gadjev I, Stone J, Gechev T (2008) Programmed cell death in plants: new insights into redox regulation and the role of hydrogen peroxide. Int. Rev. Cell Mol. Biol. 270: 87-144• Gadjev I, Vanderauwera S, Gechev T, Laloi C, Minkov I, Shulaev V, Apel K, Inzé D, Mittler• R, Van Breusegem F (2006) Transcriptomic footprints disclose specificity of reactive oxygen species signaling in Arabidopsis. Plant Physiol. 141: 436-445

Country/Organization

Bulgaria

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