Efficient Screening Techniques to Identify Mutants with Disease Resistance for Coffee and Banana

Closed for proposals

Project Type

Coordinated Research Project

Project Code

D22005

CRP

2085

Approved Date

3 July 2015

Status

Closed

Start Date

2 November 2015

Expected End Date

1 November 2020

Completed Date

17 June 2021

Description

Plant diseases pose a major threat to global food security. It is strongly predicted that the compounding factors of climate change and variability, newly emerging races of pathogens, and reliance on monocultures will increase the severity of the problem. The ability for Member States to maintain farmer’s livelihoods and to adequately feed their population with nutritious food is at risk.  Diseases affect all crops and result in massive yield losses. Traditional breeding methods are limited by narrow gene pools, and by the long time required to develop new varieties. Induced mutations offer a safe, proven and acceptable alternative route to increase the efficiency of producing crops with enhanced resistance to biotic stress. For example a search of the Mutant Variety Database (mvgs.iaea.org) shows 696 varieties when "disease" is used as a search term, and 132 varieties when "rust" is used as a keyword. Most are cereals. While progress is being made in mutation breeding of cereals owing to their ease of fertilization, excellent genetic resources and large network of researchers, less has been achieved with vegetatively propagated and perennial crops. In large part this is due to bottlenecks in mutation induction and  screening.  This CRP will focus on the development of technology packages to enhance the efficiency of generation and screening mutant populations of crop species for the improved resistance to plant diseases. Vegetatively propagated banana and the polyploid perennial arabica coffee are examples of target species where major improvements are needed to overcome existing bottlenecks .  Activities will also include methods of mutant characterization to facilitate breeding and transfer of adapted varieties to farmers.

Objectives

To provide tools and improved germplasm of coffee and banana to mitigate the effect of climate change driven crop disease outbreaks to enable sustainable food security and enhanced livelihoods in Member States.

Specific objectives

To address identified bottlenecks through development, optimization and validation of technology packages for efficient production and screening of mutant populations for disease resistance and through supportive approaches.

Impact

1. New and innovative technology packages & scientific advancement for improved mutation induction and screening of coffee and banana; transferable to other vegetative crops and trees
2. Technology dissemination & outreach
- Two protocol books & two training course manuals
- 5 journal articles published (Oct 2020); conference proceedings & abstracts
- 3 FAO/IAEA success stories with additional interviews/social media outreach
3. Human and institutional capacity building
- Ca 5 fellows, 3 PhD, 3 MSc students trained (COS, CPR, AUS, PBGL)
- Group training course ‘Mutation induction in coffee’, jointly organized and implemented by PBGL and BOKU University, Austria (3-14 October 2016)
4. Mutant germplasm:
- New coffee and banana mutant resources developed by Member States, in case of coffee, this is the first collective effort to develop mutant resources
- One TR4 resistant banana released

Relevance

o Coffee is the most important agricultural product in international trade; Leaf Rust re-emerged in Central America in 2011-2012 and by 2017 70% of farms affected; 1,7 M jobs lost and 3.2 billion USD lost income
o The Banana Fusarium Wilt TR4 disease is already present in Asia, Africa, the Middle East and Australia. The disease has spread to South America (Colombia) for the first time in 2019. Latin America is world’s most important banana exporting region.

CRP Publications

Type

Abstract

Year

2018

Description

Bado S., Maghuly F. and Laimer M. 2018. Mutation induction in Coffea spp. to counteract the impact of a changing climate. 10th ÖGMBT Annual Meeting, “10 years of life, science and molecules”. September 17-20, 2018 in Vienna, Austria.

Country/Organization

AUS

Type

Oral presentation

Year

2018

Publication URL

https://www.iaea.org/events/plant-mutation-breeding-symposium-2018

Description

Bado S., Maghuly F., Varzea V. and Laimer M. 2018. Mutagenesis of in vitro explants of Coffea arabica to induce fungal resistance. FAO/IAEA International Symposium on Plant Mutation Breeding and Biotechnology. 27-31 August 2018, Vienna, Austria.

Country/Organization

AUS

Type

Abstract

Year

2017

Description

Mustapha Akil, Affrida Abu Hassan, Norazlina Noordin, Chong Saw Peng, Zaiton Ahmad, Faiz Ahmad, Norellia Bahari, Norhayati Irwan, Nashimatul Adadiah Yahya, Nor Hafiz Talib (2017). Low-Cost Bioreactor for Rapid, Mass and Cheap Production of Plant Seedlings. In Proceedings of Nuclear Technical Conference 2017. Malaysian Nuclear Agency.

Country/Organization

MAL

Type

Oral presentation

Year

2018

Publication URL

https://www.iaea.org/events/plant-mutation-breeding-symposium-2018

Description

Noel Arrieta, Reina Cespedes. Determination of Radio-sensitivity of Coffee Arabica Var. Venecia Seeds to Gamma Ray Irradiation. FAO/IAEA International Symposium on Plant Mutation Breeding and Biotechnology. 27-31 August 2018, Vienna, Austria

Country/Organization

COS

Type

Abstract - Poster presentation

Year

2018

Publication URL

https://www.iaea.org/events/plant-mutation-breeding-symposium-2018

Description

Babita Dussoruth, Vivian Vally, 2018. Improvement of Local Dessert-Type Banana for Disease Tolerance and Agronomic Traits Through Nuclear Technique Application. FAO/IAEA International Symposium on Plant Mutation Breeding and Biotechnology. 27-31 August 2018, Vienna, Austria.

Country/Organization

MAR

Type

Article

Year

2017

Description

WANG Han*, WU Weihuai*, LI Le, LIANG Yanqiong, ZHENG Jinlong, XI Jingen,LI Rui, HUANG Xing, HE Chunping#, YI Kexian#. PCR-Based Molecular Detection of Hemileia vastatrix in Coffee. Chinese Journal of Tropical Crops, 2017,38(9):1677-1681 (Chinese)

Country/Organization

CPR

Type

Article

Year

2017

Publication URL

http://www.musalit.org/seeMore.php?id=16927

Description

Viljoen, A., Mahuku, G., Massawe, C., Ssali, R.T., Kimunye, J., Mostert, D., Ndayihanzamaso, P. and Coyne, D. 2017. Banana Diseases and Pests: Field Guide for Diagnostics and Data Collection. International Institute of Tropical Agriculture, Nairobi. 73p.

Country/Organization

SAF

Type

Abstract - Poster presentation

Year

2018

Publication URL

https://www.iaea.org/events/plant-mutation-breeding-symposium-2018

Description

Babita Dussoruth, Joanna Jankowicz-Cieslak, 2018. Irradiation as Means for Increasing Genetic Diversity in Banana: The Need to Carry Out Radiosensitivity Tests on In Vitro Cultures at Appropriate Stage of Growth. FAO/IAEA International Symposium on Plant Mutation Breeding and Biotechnology. 27-31 August 2018, Vienna, Austria.

Country/Organization

MAR; FAO/IAEA

Type

Abstract - Poster presentation

Year

2018

Publication URL

https://www.iaea.org/events/plant-mutation-breeding-symposium-2018

Description

Noel Arrieta, Reina Cespedes, Miguel Barquero. Radiosensitivity Test on Gamma Irradiated Coffea arabica Var. Venecia In Vitro Zygotic Embryos. FAO/IAEA International Symposium on Plant Mutation Breeding and Biotechnology, 27-31 August 2018, Vienna, Austria

Country/Organization

COS

Type

Abstract

Year

2018

Description

Bado S., Maghuly F. and Laimer M. 2018. Mutation induction in Coffea spp. to counteract the impact of a changing climate. 10th ÖGMBT Annual Meeting, “10 years of life, science and molecules”. September 17-20, 2018 in Vienna, Austria.

Country/Organization

AUS

Type

Abstract

Year

2017

Description

Mustapha Akil, Affrida Abu Hassan, Norazlina Noordin, Chong Saw Peng, Zaiton Ahmad, Faiz Ahmad, Norellia Bahari, Norhayati Irwan, Nashimatul Adadiah Yahya, Nor Hafiz Talib (2017). Low-Cost Bioreactor for Rapid, Mass and Cheap Production of Plant Seedlings. In Proceedings of Nuclear Technical Conference 2017. Malaysian Nuclear Agency.

Country/Organization

MAL

Type

Abstract - Poster presentation

Year

2018

Publication URL

https://www.iaea.org/events/plant-mutation-breeding-symposium-2018

Description

Babita Dussoruth, Joanna Jankowicz-Cieslak, 2018. Irradiation as Means for Increasing Genetic Diversity in Banana: The Need to Carry Out Radiosensitivity Tests on In Vitro Cultures at Appropriate Stage of Growth. FAO/IAEA International Symposium on Plant Mutation Breeding and Biotechnology. 27-31 August 2018, Vienna, Austria.

Country/Organization

MAR; FAO/IAEA

Type

Article

Year

2017

Description

WANG Han*, WU Weihuai*, LI Le, LIANG Yanqiong, ZHENG Jinlong, XI Jingen,LI Rui, HUANG Xing, HE Chunping#, YI Kexian#. PCR-Based Molecular Detection of Hemileia vastatrix in Coffee. Chinese Journal of Tropical Crops, 2017,38(9):1677-1681 (Chinese)

Country/Organization

CPR

Type

Abstract - Poster presentation

Year

2018

Publication URL

https://www.iaea.org/events/plant-mutation-breeding-symposium-2018

Description

Babita Dussoruth, Vivian Vally, 2018. Improvement of Local Dessert-Type Banana for Disease Tolerance and Agronomic Traits Through Nuclear Technique Application. FAO/IAEA International Symposium on Plant Mutation Breeding and Biotechnology. 27-31 August 2018, Vienna, Austria.

Country/Organization

MAR

Type

Article

Year

2017

Publication URL

http://www.musalit.org/seeMore.php?id=16927

Description

Viljoen, A., Mahuku, G., Massawe, C., Ssali, R.T., Kimunye, J., Mostert, D., Ndayihanzamaso, P. and Coyne, D. 2017. Banana Diseases and Pests: Field Guide for Diagnostics and Data Collection. International Institute of Tropical Agriculture, Nairobi. 73p.

Country/Organization

SAF

Type

Abstract - Poster presentation

Year

2018

Publication URL

https://www.iaea.org/events/plant-mutation-breeding-symposium-2018

Description

Noel Arrieta, Reina Cespedes, Miguel Barquero. Radiosensitivity Test on Gamma Irradiated Coffea arabica Var. Venecia In Vitro Zygotic Embryos. FAO/IAEA International Symposium on Plant Mutation Breeding and Biotechnology, 27-31 August 2018, Vienna, Austria

Country/Organization

COS

Type

Oral presentation

Year

2018

Publication URL

https://www.iaea.org/events/plant-mutation-breeding-symposium-2018

Description

Bado S., Maghuly F., Varzea V. and Laimer M. 2018. Mutagenesis of in vitro explants of Coffea arabica to induce fungal resistance. FAO/IAEA International Symposium on Plant Mutation Breeding and Biotechnology. 27-31 August 2018, Vienna, Austria.

Country/Organization

AUS

Type

Thesis

Year

2018

Description

Inducción de mutaciones por irradiación gamma en semillas y embriones cigóticos de Coffea arabica VAR. Venecia. (Thesis). Author – Céspedes-Acosta, R. Reviewers – Arrieta-Espinoza, N., Barquero-Miranda, M. & Abdelnour-Esquivel, A.

Country/Organization

COS

Type

Thesis

Year

2018

Description

Inducción de mutaciones por irradiación gamma en semillas y embriones cigóticos de Coffea arabica VAR. Venecia. (Thesis). Author – Céspedes-Acosta, R. Reviewers – Arrieta-Espinoza, N., Barquero-Miranda, M. & Abdelnour-Esquivel, A.

Country/Organization

COS

Type

Oral presentation

Year

2018

Publication URL

https://www.iaea.org/events/plant-mutation-breeding-symposium-2018

Description

Noel Arrieta, Reina Cespedes. Determination of Radio-sensitivity of Coffee Arabica Var. Venecia Seeds to Gamma Ray Irradiation. FAO/IAEA International Symposium on Plant Mutation Breeding and Biotechnology. 27-31 August 2018, Vienna, Austria

Country/Organization

COS

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