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The development of better system for dose distribution within an irradiation container and the development of an accurate dose-response curve for the target insect using precise dosimetry is a prerequisite of any programmes releasing sterile insects. This manual describes the operational procedures to develop dose maps by scanning Gafchromic film and the calibration of the system, to be used in the insect irradiation process for SIT programmes. The manual is also available in Spanish/Español.