Manuals and protocols

Guideline on Phytosanitary Procedures for Area-Wide Management of Fruit Fly Pests

International Guideline for Transboundary Shipments of Irradiated Sterile Insects

Guidelines for mark-release-recapture procedures of Aedes mosquitoess_v2.0.

The protocols presented in these guidelines are the results of lessons from collaborations with Member States preparing SIT pilot trials against Aedes species. They were developed to expose the problems encountered in order to provide useful insights to develop MRR protocols that should be adapted to each situation and research questions.

Guidelines for Biosafety and Biosecurity in Mosquito Rearing Facilities_Version 2.0

The present guidelines refer to a mosquito rearing facility which is suitable for work with uninfected mosquito vectors including: 1) mosquitoes that are already present in the local geographic region, and 2) exotic mosquitoes that upon escape would be inviable or become only temporarily established in areas not having active vector borne disease transmission.

Directrices generales para facilitar la apertura de mercados internacionales de frutas y hortalizas hospedantes de moscas de la fruta con base en las Normas Internacionales de Medidas Fitosanitarias

Guidelines for Mass Rearing and Irradiation of Drosophila suzukii for Sterile Insect Technique Application Version 1.0

General guidelines to facilitate the opening of international markets for fruits and vegetables that are fruit fly hosts based on International Standards for Phytosanitary Measures

Dosimetry for SIT: Standard Operating Procedures for Gafchromic™ Film Dosimetry System for Gamma Radiation v1.0

This SOP brings together in one place a description of the components of the Gafchromic™ dosimetry system for Gamma Radiation, the procedure for its characterization and its application to process validation and process control, together with references to the relevant standards. It provides a readily available source of information that can be accessed by both research workers and production facility managers. The SOP is also available in Spanish.

Manual de Control de Calidad del Producto en la Cría masiva y Liberación de Moscas de la Fruta Estériles, (traducción de la Versión 7.0 de 2019)

Dosimetry for SIT: Standard Operating Procedures for Gafchromic™ Film Dosimetry System for Low Energy X Radiation v1.0

This SOP brings together in one place a description of the components of the Gafchromic™ dosimetry system for Low Energy X Radiation, the procedure for its characterization, and its application to process validation and process control, together with references to the relevant standards. It provides a readily available source of information that can be accessed by both research workers and production facility managers. The SOP is also available in Spanish

Guidelines for Irradiation of Mosquito Pupae in Sterile Insect Technique Programmes

Dose mapping by scanning Gafchromic® film to measure the absorbed dose of insects during their sterilization

Harmonized identification guideline of tefritids that might be considered of economic and quarantine importance in Latin America and the Caribbean

The correct identification of endemic fruit fly pests is fundamental for the design of effective integrated pest management programmes including those that apply the sterile insect technique (SIT). Also, for the enforcement of emergency action plans when
incursions of fruit fly quarantine species occur in an area. This manual is aimed at offering National Plant protection Organizations (NPPOs) with a harmonized guide that can support them in the continues
battle against fruit fly pests affecting food security and safety in the countries (manual only available in Spanish).

Guidance framework for testing the sterile insect technique as a vector control tool against Aedes-borne diseases

This document is intended to be a comprehensive guide for programme managers tasked with recommending a “go/no-go” decision on testing, full deployment and scale-up of the sterile insect technique (SIT) in regions of the world affected by diseases transmitted by Aedes mosquitoes. However, the authors hope that the material presented herein will be used more widely—by scientists, decision makers, review groups and others.

Guidelines for Blood Collection, Processing and Quality Control for Tsetse Rearing Insectaries, Version 2.0

This document is intended for use in laboratories and institutions that maintain colonies of tsetse flies. It describes the procedures for the collection of animal blood in the abattoir, decontamination through ionizing radiation, preservation and storage, quality control assurance and processing of the blood into diet for feeding tsetse flies.

Guidelines for mass rearing of Aedes mosquitoes_v1.0

The number and production capacity of mass-rearing insectaries for Aedes mosquitoes is increasing as the SIT technology is being validated in field pilot projects. These guidelines have been developed to provide a description of procedures required for Aedes aegypti and Aedes albopictus mass-rearing. It is a summary of necessary steps of larval and adult mass-rearing as used at the FAO/IAEA. This guide will be continuously updated considering the improvements brought to the mass-rearing of mosquitoes.

Product Quality Control for Sterile Mass-Reared and Released Tephritid Fruit Flies, Version 7.0.

FAO/IAEA/USDA. FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture. IAEA, Vienna, Austria (2019).

Use of Entomopathogenic Fungi for Fruit Fly Control in Area-Wide SIT Programmes

Standard Operating Procedures for Detection and Identification of Trypanosome Species in Tsetse Flies Version 1.0

The manual provides an easy-to-use work flow that combines several different PCR-based methods that can be used for trypanosome detection and identification in field-caught tsetse flies and/or host blood samples collected from livestock and wildlife animals.

Spreadsheet for Designing Aedes Mosquito Mass-Rearing and Release Facilities Version 1.0