IPC Publications

Plan Rector Regional para la Aplicación del Manejo Integrado de Moscas de la Fruta

The Regional Master Plan for the Application of Integrated Fruit Fly Management based on the Sterile Insect Technique (SIT), addresses the problems caused by fruit flies of economic and quarantine importance, with a special emphasis on the South American fruit fly (Anastrepha fraterculus) and the Mediterranean fruit fly (Ceratitis capitata). The plan therefore proposes close collaboration among South American countries through the creation of a Regional Commission to coordinate fruit fly pest management activities.

Guideline on Phytosanitary Procedures for Area-Wide Management of Fruit Fly Pests

This guideline describes the most widely used classic and modern phytosanitary procedures for the management of fruit flies that infest fruits and vegetables and that are of quarantine and economic importance, specifically species of the genera Anastrepha, Bactrocera, Ceratitis, Dacus, Rhagoletis and Zeugodacus. Pest management can be aimed at the native species of fruit flies or to prevent the introduction, establishment and spread of those non-native species.

International Guideline for Transboundary Shipments of Irradiated Sterile Insects

This document contains guidelines for transboundary shipment and importation of irradiated sterile insects (either as a consignment in transit or for entry in the country of destination). Producers/shippers and importers of sterile insects may be private businesses as well as government, parastatal, joint venture or international organizations.

Guidelines for mark-release-recapture procedures of Aedes mosquitoess_v2.0.

The protocols presented in these guidelines are the results of lessons from collaborations with Member States preparing SIT pilot trials against Aedes species. They were developed to expose the problems encountered in order to provide useful insights to develop MRR protocols that should be adapted to each situation and research questions.

Guidelines for Biosafety and Biosecurity in Mosquito Rearing Facilities_Version 2.0

The present guidelines refer to a mosquito rearing facility which is suitable for work with uninfected mosquito vectors including: 1) mosquitoes that are already present in the local geographic region, and 2) exotic mosquitoes that upon escape would be inviable or become only temporarily established in areas not having active vector borne disease transmission.

Directrices generales para facilitar la apertura de mercados internacionales de frutas y hortalizas hospedantes de moscas de la fruta con base en las Normas Internacionales de Medidas Fitosanitarias

Directrices Generales para Facilitar la Apertura de Mercados Internacionales de Frutas y Hortalizas. El objetivo de este documento es profundizar en el uso de las normas internacionales para facilitar el comercio internacional de frutas y hortalizas frescas. Conocido es que muchos de estos productos son hospedantes de moscas de la fruta.

Guidelines for Mass Rearing and Irradiation of Drosophila suzukii for Sterile Insect Technique Application Version 1.0

The present document describes the standard mass rearing protocols and sterilization procedures currently used at the IPCL. In the first part of the document, we describe the mass rearing oviposition system used at the IPCL and detail the different steps necessary to maintain a SWD laboratory colony, in particular egg collection, larval rearing, and pupae collection. In the second part, we present the pupal irradiation procedure, as well as the method to measure adult sterility level.

General guidelines to facilitate the opening of international markets for fruits and vegetables that are fruit fly hosts based on International Standards for Phytosanitary Measures

The key to opening markets in the World Trade Organization (WTO) Agreement on the Application of Sanitary and Phytosanitary Measures (the SPS Agreement) and the International Plant Protection Convention (IPPC) framework, is to focus on the pest risk, and especially on all the factors contributing to risk and mitigating risk. There is often a tendency to begin with legacy concepts and requirements that were based on assumptions of high risk rather than begin with a fresh view of the evidence and an objective analysis of the risk.

Dosimetry for SIT: Standard Operating Procedures for Gafchromic™ Film Dosimetry System for Gamma Radiation v1.0

This SOP brings together in one place a description of the components of the Gafchromic™ dosimetry system for Gamma Radiation, the procedure for its characterization and its application to process validation and process control, together with references to the relevant standards. It provides a readily available source of information that can be accessed by both research workers and production facility managers. The SOP is also available in Spanish.

Manual de Control de Calidad del Producto en la Cría masiva y Liberación de Moscas de la Fruta Estériles, (traducción de la Versión 7.0 de 2019)

FAO/IAEA/USDA. FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture. IAEA, Vienna, Austria (2022)

Dosimetry for SIT: Standard Operating Procedures for Gafchromic™ Film Dosimetry System for Low Energy X Radiation v1.0

This SOP brings together in one place a description of the components of the Gafchromic™ dosimetry system for Low Energy X Radiation, the procedure for its characterization, and its application to process validation and process control, together with references to the relevant standards. It provides a readily available source of information that can be accessed by both research workers and production facility managers. The SOP is also available in Spanish

Guidelines for Irradiation of Mosquito Pupae in Sterile Insect Technique Programmes

This publication is intended as guidance for the irradiation of the pupal stage of Aedes aegypti, Aedes albopictus and Anopheles arabiensis, for routine studies on the biological effects of radiation exposures, in particular, irradiation induced sterility in male (and female) mosquitoes.

Dose mapping by scanning Gafchromic® film to measure the absorbed dose of insects during their sterilization

The development of better system for dose distribution within an irradiation container and the development of an accurate dose-response curve for the target insect using precise dosimetry is a prerequisite of any programmes releasing sterile insects. This manual describes the operational procedures to develop dose maps by scanning Gafchromic film and the calibration of the system, to be used in the insect irradiation process for SIT programmes. The manual is also available in  Spanish/Español.

Harmonized identification guideline of tefritids that might be considered of economic and quarantine importance in Latin America and the Caribbean

The correct identification of endemic fruit fly pests is fundamental for the design of effective integrated pest management programmes including those that apply the sterile insect technique (SIT). Also, for the enforcement of emergency action plans when
incursions of fruit fly quarantine species occur in an area. This manual is aimed at offering National Plant protection Organizations (NPPOs) with a harmonized guide that can support them in the continues
battle against fruit fly pests affecting food security and safety in the countries (manual only available in Spanish).

Guidance framework for testing the sterile insect technique as a vector control tool against Aedes-borne diseases

This document is intended to be a comprehensive guide for programme managers tasked with recommending a “go/no-go” decision on testing, full deployment and scale-up of the sterile insect technique (SIT) in regions of the world affected by diseases transmitted by Aedes mosquitoes. However, the authors hope that the material presented herein will be used more widely—by scientists, decision makers, review groups and others.

Guidelines for Blood Collection, Processing and Quality Control for Tsetse Rearing Insectaries, Version 2.0

This document is intended for use in laboratories and institutions that maintain colonies of tsetse flies. It describes the procedures for the collection of animal blood in the abattoir, decontamination through ionizing radiation, preservation and storage, quality control assurance and processing of the blood into diet for feeding tsetse flies.

Guidelines for mass rearing of Aedes mosquitoes_v1.0

The number and production capacity of mass-rearing insectaries for Aedes mosquitoes is increasing as the SIT technology is being validated in field pilot projects. These guidelines have been developed to provide a description of procedures required for Aedes aegypti and Aedes albopictus mass-rearing. It is a summary of necessary steps of larval and adult mass-rearing as used at the FAO/IAEA. This guide will be continuously updated considering the improvements brought to the mass-rearing of mosquitoes.

Product Quality Control for Sterile Mass-Reared and Released Tephritid Fruit Flies, Version 7.0.

FAO/IAEA/USDA. FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture. IAEA, Vienna, Austria (2019).

Use of Entomopathogenic Fungi for Fruit Fly Control in Area-Wide SIT Programmes

Standard Operating Procedures for Detection and Identification of Trypanosome Species in Tsetse Flies Version 1.0

The manual provides an easy-to-use work flow that combines several different PCR-based methods that can be used for trypanosome detection and identification in field-caught tsetse flies and/or host blood samples collected from livestock and wildlife animals.