Integration of the SIT with Biocontrol for Greenhouse Insect Pest Management

Closed for proposals

Project Type

Coordinated Research Project

Project Code

D43003

CRP

2116

Approved Date

14 September 2016

Status

Closed

Start Date

17 March 2017

Expected End Date

16 March 2023

Completed Date

12 July 2023

Participating Countries

Argentina
Brazil
Canada
Chile
Egypt
France
Guatemala
India
Iran (Islamic Republic of)
Italy
Mexico
New Zealand
Pakistan

Description

This CRP will develop and evaluate SIT for insect pests in greenhouses. The CRP will focus on three groups of species that are disruptive to current biocontrol practices, or are expected to become serious pest when invading new areas. The three groups are Drosophila suzukii, Heilcoverpa / Spodoptera spp. and Tuta absoluta / Neoleucinodes elegantalis. The background information required for SIT, such as mass-rearing and radiation biology, has been developed for these greenhouse pests to a variable extent. However, for all of these groups the application in confined areas still needs to be refined and evaluated.

Objectives

The overall objective is to advance development and implementation of SIT for integration with other biocontrol in greenhouses.

Specific objectives

To develop SIT and inherited sterility for Tuta absoluta and Neoleucinodes elegantalis

To adapt SIT and inherited sterility for Spodoptera/Helicoverpa species for confined cropping systems

To develop SIT for Drosophila suzukii

To develop SIT and inherited sterility for Tuta absoluta and Neoleucinodes elegantalis

To adapt SIT and inherited sterility for Spodoptera/Helicoverpa species for confined cropping systems

To develop SIT for Drosophila suzukii

Impact

1. Guidelines for D suzukii mass rearing and irradiation protocols https://www.iaea.org/sites/default/files/massrearing-and-irradiation-swd... provides tools and knowledge for member states.
2. Quality control parameters guidelines under development will provide knowledge and tools for member states.
3. Knowing the effect of biological control agents gives new residue-free options for D. suzukii suppression.
4. Population models and molecular markers provide guidance on numbers of sterile insects and BCAs for D. suzukii management.
5. Desirable phenotypic traits to improve mating success of mass-reared sterilized insects were identified.
6. Dose response of parental, and subsequent sterilization of F1, has been established for Tuta absoluta, Helicoverpa armigera and Spodoptera littoralis.
7. Diets have been developed for T. absoluta, H. armigera and S. littoralis mass rearing.
8. No asexually reproducing populations of T. absoluta have been detected in Argentina meaning that SIT remains viable for Argentinian populations.
9. The presence of Wolbachia in T. absoluta from India (Supergroup A and B) and Argentina (Supergroup B) means that cytoplasmic incompatibility is a possible tool for T. absoluta management.
10. Biocontrol agents like Chrysoperla sp., Tupiocoris sp. Trichogramma sp., have been validated as viable control agents for T. absoluta and S. littoralis.

The results achieved during this CRP have now made it possible to manage Drosophila suzukii and Tuta absoluta with the sterile insect technique and with biological control agents. Key diet development and dose response steps have been achieved to realize the implementation of the sterile insect technique and inherited sterility technique for future Helicoverpa armigera and Spodoptera littoralis management.

Relevance

The CRP pioneered the development of the sterile insect technique for Drosophila suzukii and Tuta absoluta. The results of the research undertaken during the CRP have provided novel information regarding the dose response, mass rearing diet and insect collection and the use of biological control agents for population suppression of Drosophila suzukii and Tuta absoluta. Furthermore, the methodologies have been transferred to Helicoverpa armigera and Spodoptera littoralis. Specific mass rearing and irradiation recommendations have been provided in an IAEA guideline document. Semi-field trials have validated the SIT and BCA strategies as viable options for D. suzukii and T. absoluta. Wolbachia was identified from Tuta populations, paving the way for the development of management tools utilizing cytoplasmic incompatibility.

CRP Publications

Type

Research article

Year

2019

Publication URL

https://www.divecosys.org/content/download/4883/38640/version/1/file/2018_Mansou…

Description

Occurrence, biology, natural enemies and management of Tuta absoluta in Africa

Country/Organization

Université de Carthage, Institut Supérieur des Études Préparatoires en Biologie Géologie (ISEP-BG) / Tunisia Year: 2019

Type

Research article

Year

2019

Publication URL

https://doi.org/10.3958/059.044.0307

Description

Effect of irradiation on quality and fertility parameters of Drosophila suzukii in Mexico

Country/Organization

México, SENASICA-CNRF, Carretera Federal México-Pachuca Km 37.5 C.P. 55740, Tecámac, Estado de México Departamento de Fitossanidade

Type

Reserch article

Year

2019

Publication URL

https://link.springer.com/content/pdf/10.1007/s10526-018-09914-0.pdf

Description

Augmentative releases of Trichopria drosophilae for the suppression of early season Drosophila suzukii populations

Country/Organization

USA / Italy - Department of Horticulture, Oregon State University / Technology Transfer Centre, Fondazione Edmund Mach, Italy

Type

Research article

Year

2019

Publication URL

https://doi.org/10.1111/jen.12563

Description

Radiation effects on Drosophila suzukii (Diptera: Drosophilidae) reproductive behaviour

Country/Organization

Brazil, Departamento de Fitossanidade, Universidade Federal de Pelotas, Pelotas, RS, Brazil. Type: Research article

Type

Reserch article

Year

2022

Description

Polyandry is an important reproductive trait of the invasive Drosophila suzukii

Country/Organization

Italy, University of Pavia, Department of Biology and Biotechnology

Type

Reserch article

Year

2020

Publication URL

https://academic.oup.com/jee/advance-article/doi/10.1093/jee/toaa022/5738996

Description

Mass Rearing, Quality Parameters, and Bioconversion in Drosophila suzukii (Diptera: Drosophilidae) for Sterile Insect Technique Purposes

Country/Organization

Mexico / Programa Moscafrut SADER-SENASICA, Subdirección de Desarrollo de Métodos, Camino a Cacaotales s/n 30860, Metapa de Domínguez, Chiapas, México

Type

Research article

Year

2017

Publication URL

https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0180821&t…

Description

The sterile insect technique for the management of the spotted wing drosophila, Drosophila suzukii: Establishing the optimum irradiation dose

Country/Organization

Canada / Austria - Institut de recherche et de de´veloppement en agroenvironnement, Saint-Bruno-de-Montarville, Que´bec, - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture

Type

Research article

Year

2018

Description

Effect of gamma irradiation on the susceptibility of the cotton leaf worm, Spodoptera littoralis (Bosid.) (Lepidoptera: Noctuidae) to the infection with nucleopolyhedrosis virus

Country/Organization

Biological Application Department, Nuclear research center, Atomic Energy Authority, Cairo, Egypt and Faculty of Agriculture, Department of Economic Entomology and pesticide, Cairo university Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture

Type

Research article

Year

2019

Publication URL

https://link.springer.com/content/pdf/10.1007/s10526-018-09914-0.pdf

Description

Augmentative releases of Trichopria drosophilae for the suppression of early season Drosophila suzukii populations

Country/Organization

USA / Italy - Department of Horticulture, Oregon State University / Technology Transfer Centre, Fondazione Edmund Mach, Italy

Type

Institute for Insect Biotechnology, Justus-Liebig-University Giessen, Giessen, Germany

Year

2018

Publication URL

https://onlinelibrary.wiley.com/doi/10.1111/jen.12469

Description

Environmentally sustainable pest control options for Drosophila suzukii

Country/Organization

Germany

Type

Research article

Year

2021

Publication URL

https://doi.org/10.1038/s41598-021-90034-8

Description

The pest kill rate of thirteen natural enemies as aggregate evaluation criterion of their biological control potential of Tuta absoluta. Scientific Reports. 11, 10756.

Country/Organization

Argentina - Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina.

Type

Reserch article

Year

2019

Publication URL

https://doi.org/10.3390/insects10120448

Description

Mass-Rearing of Drosophila suzukii for Sterile Insect Technique Application: Evaluation of Two Oviposition Systems

Country/Organization

Austria / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture

Type

Reserch article

Year

2019

Publication URL

https://link.springer.com/content/pdf/10.1007/s10526-018-09914-0.pdf

Description

Augmentative releases of Trichopria drosophilae for the suppression of early season Drosophila suzukii populations

Country/Organization

USA / Italy - Department of Horticulture, Oregon State University / Technology Transfer Centre, Fondazione Edmund Mach, Italy

Type

Reserch article

Year

2017

Publication URL

https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0180821&t…

Description

The sterile insect technique for the management of the spotted wing drosophila, Drosophila suzukii: Establishing the optimum irradiation dose

Country/Organization

Canada / Austria - Institut de recherche et de de´veloppement en agroenvironnement, Saint-Bruno-de-Montarville, Que´bec, - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture

Type

Reserch article

Year

2019

Publication URL

https://www.divecosys.org/content/download/4883/38640/version/1/file/2018_Mansou…

Description

Occurrence, biology, natural enemies and management of Tuta absoluta in Africa

Country/Organization

Université de Carthage, Institut Supérieur des Études Préparatoires en BiologieGéologie (ISEP-BG) / Tunisia

Type

Reserch article

Year

2018

Publication URL

https://onlinelibrary.wiley.com/doi/10.1111/jen.12469

Description

Environmentally sustainable pest control options for Drosophila suzukii

Country/Organization

Germany Institute for Insect Biotechnology, Justus-Liebig-University Giessen, Giessen, Germany

Type

Reserch article

Year

2019

Publication URL

https://doi.org/10.3390/insects10120448

Description

Mass-Rearing of Drosophila suzukii for Sterile Insect Technique Application: Evaluation of Two Oviposition Systems

Country/Organization

Austria / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture

Type

Université de Carthage, Institut Supérieur des Études Préparatoires en BiologieGéologie (ISEP-BG) / Tunisia

Year

2019

Publication URL

https://www.divecosys.org/content/download/4883/38640/version/1/file/2018_Mansou…

Description

Occurrence, biology, natural enemies and management of Tuta absoluta in Africa

Type

Research article

Year

2018

Publication URL

https://doi.org/10.1093/jee/tox349

Description

Effects of irradiation dose on sterility induction and quality parameters of Drosophila suzukii (Diptera: Drosophilidae)

Country/Organization

Brazil, Departamento de Fitossanidade, Universidade Federal de Pelotas, Pelotas, RS, Brazil. Type: Research article

Type

_Research article

Year

2021

Publication URL

https://aj.tubitak.gov.tr/agriculture/issues/tar-21-45-1/tar-45-1-10-2006-12.pdf

Description

Analysis of parthenogenesis and molecular detection of reproductive symbionts in Tuta absoluta (Lepidoptera: Gelechiidae) from Argentina. Entomologia Experimentalis et applicata.

Country/Organization

Argentina - Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina.

Type

Research article

Year

2018

Publication URL

https://onlinelibrary.wiley.com/doi/10.1111/jen.12469

Description

Environmentally sustainable pest control options for Drosophila suzukii

Country/Organization

Germany Institute for Insect Biotechnology, Justus-Liebig-University Giessen, Giessen, Germany

Type

Research article

Year

2018

Publication URL

https://bulletin.incas.ro/

Description

Development of ecofriendly sterile insect technique for agriculturally important insect pests

Country/Organization

India- Nuclear Agriculture & Biotechnology Division, Bhabha Atomic Research Centre

Type

Research article

Year

2021

Description

Pathogenicity and virulence of Isaria javanica, Metarhizium anisopliae, and Beauveria bassiana strains for control of Drosophila suzukii (Matsumura)

Country/Organization

Organization: Mexico / Programa Moscafrut SADER-SENASICA, Subdirección de Desarrollo de Métodos, Camino a Cacaotales s/n 30860, Metapa de Domínguez, Chiapas, México

Type

Reserch article

Year

2019

Publication URL

https://doi.org/10.1371/journal.pone.0226582

Description

Irradiation dose response under hypoxia for the application of the sterile insect technique in Drosophila suzukii

Country/Organization

Austria / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture

Type

Reserch article

Year

2019

Publication URL

https://link.springer.com/content/pdf/10.1007/s10526-018-09914-0.pdf

Description

Augmentative releases of Trichopria drosophilae for the suppression of early season Drosophila suzukii populations

Country/Organization

USA / Italy - Department of Horticulture, Oregon State University / Technology Transfer Centre, Fondazione Edmund Mach, Italy

Type

Reserch article

Year

2018

Publication URL

https://link.springer.com/content/pdf/10.1007/s10340-017-0944-y.pdf

Description

Sterile insect technique and Wolbachia symbiosis as potential tools for the control of the invasive species Drosophila suzukii

Country/Organization

Austria - France / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture - Université de Rennes

Type

Scientific presentation

Year

2022

Description

Unravelling the reproductive biology traits of Drosophila suzukii as a basis for specific and long-term control of this species

Country/Organization

Italy, University of Pavia, Department of Biology and Biotechnology

Type

Scientific poster

Year

2022

Publication URL

https://www.11isffei.com/program

Country/Organization

Austria, Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture

Type

Reserch article

Year

2019

Publication URL

https://doi.org/10.1371/journal.pone.0226582

Country/Organization

Austria / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture

Type

Reserch article

Year

2019

Publication URL

https://doi.org/10.3390/insects10120448

Description

Mass-Rearing of Drosophila suzukii for Sterile Insect Technique Application: Evaluation of Two Oviposition Systems

Country/Organization

Austria / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture

Type

Reserch article

Year

2019

Publication URL

https://link.springer.com/content/pdf/10.1007/s10340-017-0944-y.pdf

Description

Sterile insect technique and Wolbachia symbiosis as potential tools for the control of the invasive species Drosophila suzukii

Country/Organization

Austria - France / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture - Université de Rennes

Type

Research article

Year

2020

Publication URL

https://ejbpc.springeropen.com/articles/10.1186/s41938-019-0201-1

Description

Increasing the efficacy of the cotton leaf worm Spodoptera littoralis nucleopolyhedrosis virus using certain essential oils

Country/Organization

Biological Application Department, Nuclear research center, Atomic Energy Authority, Cairo, Egypt joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture

Type

Research article

Year

2018

Publication URL

https://www.informaticsjournals.com/index.php/jbc/article/view/23115

Description

Molecular characterisation of tomato leaf miner Tuta absoluta populations obtained from different geographical locations of India.

Country/Organization

India- Nuclear Agriculture & Biotechnology Division, Bhabha Atomic Research Centre

Type

Reserch article

Year

2018

Publication URL

https://link.springer.com/content/pdf/10.1007/s10340-017-0944-y.pdf

Description

Sterile insect technique and Wolbachia symbiosis as potential tools for the control of the invasive species Drosophila suzukii

Country/Organization

Austria - France / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture - Université de Rennes

Type

Reserch article

Year

2017

Publication URL

https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0180821&t…

Description

The sterile insect technique for the management of the spotted wing drosophila, Drosophila suzukii: Establishing the optimum irradiation dose

Country/Organization

Canada / Austria - Institut de recherche et de de´veloppement en agroenvironnement, Saint-Bruno-de-Montarville, Que´bec, - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture

Type

Université de Carthage, Institut Supérieur des Études Préparatoires en BiologieGéologie (ISEP-BG) / Tunisia

Year

2019

Publication URL

https://www.divecosys.org/content/download/4883/38640/version/1/file/2018_Mansou…

Description

Occurrence, biology, natural enemies and management of Tuta absoluta in Africa

Type

Research article

Year

2021

Description

Effect of low cost diet on the cotton leaf worm , Spodoptera littoralis nucleopolyhedrosis virus pathogenicity and sterile insect technique

Country/Organization

Biological Application Department, Nuclear research center, Atomic Energy Authority, Cairo, Egypt , Department of plant protection Faculty of Agriculture, Fayoum University joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture

Type

Research article

Year

2021

Publication URL

https://doi.org/10.1111/jen.12894

Description

Hypoxia combined with chilling maintains the quality of irradiated Drosophila flies: a simulated shipment experiment

Country/Organization

Austria / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture

Type

Guideline

Year

2022

Publication URL

https://www.iaea.org/sites/default/files/massrearing-and-irradiation-swd.pdf

Description

Guidelines for Mass Rearing and Irradiation of Drosophila suzukii for Sterile Insect Technique Application

Country/Organization

FAO/IAEA - IPCL

Type

Reserch article

Year

2019

Publication URL

https://doi.org/10.1371/journal.pone.0226582

Country/Organization

Austria / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture

Type

Reserch article

Year

2020

Publication URL

https://academic.oup.com/jee/advance-article/doi/10.1093/jee/toaa022/5738996

Description

Mass Rearing, Quality Parameters, and Bioconversion in Drosophila suzukii (Diptera: Drosophilidae) for Sterile Insect Technique Purposes

Country/Organization

Mexico / Programa Moscafrut SADER-SENASICA, Subdirección de Desarrollo de Métodos, Camino a Cacaotales s/n 30860, Metapa de Domínguez, Chiapas, México

Type

Reserch article

Year

2020

Publication URL

https://academic.oup.com/jee/advance-article/doi/10.1093/jee/toaa022/5738996

Description

Mass Rearing, Quality Parameters, and Bioconversion in Drosophila suzukii (Diptera: Drosophilidae) for Sterile Insect Technique Purposes

Country/Organization

Mexico / Programa Moscafrut SADER-SENASICA, Subdirección de Desarrollo de Métodos, Camino a Cacaotales s/n 30860, Metapa de Domínguez, Chiapas, México

Type

Research article

Year

2019

Publication URL

https://doi.org/10.1371/journal.pone.0226582

Description

Mass-Rearing of Drosophila suzukii for Sterile Insect Technique Application: Evaluation of Two Oviposition Systems

Country/Organization

Austria / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture

Type

Reserch article

Year

2017

Publication URL

https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0180821&t…

Description

The sterile insect technique for the management of the spotted wing drosophila, Drosophila suzukii: Establishing the optimum irradiation dose

Country/Organization

Canada / Austria - Institut de recherche et de de´veloppement en agroenvironnement, Saint-Bruno-de-Montarville, Que´bec, - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture

Type

Institute for Insect Biotechnology, Justus-Liebig-University Giessen, Giessen, Germany

Year

2018

Publication URL

https://onlinelibrary.wiley.com/doi/10.1111/jen.12469

Description

Environmentally sustainable pest control options for Drosophila suzukii

Country/Organization

Germany

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