Project Type
Coordinated Research Project
Project Code
D43003
CRP
2116
Approved Date
14 September 2016
Expected End Date
16 March 2023
Completed Date
12 July 2023
Participating Countries
Argentina
Brazil
Canada
Chile
Egypt
France
Guatemala
India
Iran (Islamic Republic of)
Italy
Mexico
New Zealand
Pakistan
Description
This CRP will develop and evaluate SIT for insect pests in greenhouses. The CRP will focus on three groups of species that are disruptive to current biocontrol practices, or are expected to become serious pest when invading new areas. The three groups are Drosophila suzukii, Heilcoverpa / Spodoptera spp. and Tuta absoluta / Neoleucinodes elegantalis. The background information required for SIT, such as mass-rearing and radiation biology, has been developed for these greenhouse pests to a variable extent. However, for all of these groups the application in confined areas still needs to be refined and evaluated.
Objectives
The overall objective is to advance development and implementation of SIT for integration with other biocontrol in greenhouses.
Specific objectives
To develop SIT and inherited sterility for Tuta absoluta and Neoleucinodes elegantalis
To adapt SIT and inherited sterility for Spodoptera/Helicoverpa species for confined cropping systems
To develop SIT for Drosophila suzukii
To develop SIT and inherited sterility for Tuta absoluta and Neoleucinodes elegantalis
To adapt SIT and inherited sterility for Spodoptera/Helicoverpa species for confined cropping systems
To develop SIT for Drosophila suzukii
Impact
1. Guidelines for D suzukii mass rearing and irradiation protocols https://www.iaea.org/sites/default/files/massrearing-and-irradiation-swd... provides tools and knowledge for member states.
2. Quality control parameters guidelines under development will provide knowledge and tools for member states.
3. Knowing the effect of biological control agents gives new residue-free options for D. suzukii suppression.
4. Population models and molecular markers provide guidance on numbers of sterile insects and BCAs for D. suzukii management.
5. Desirable phenotypic traits to improve mating success of mass-reared sterilized insects were identified.
6. Dose response of parental, and subsequent sterilization of F1, has been established for Tuta absoluta, Helicoverpa armigera and Spodoptera littoralis.
7. Diets have been developed for T. absoluta, H. armigera and S. littoralis mass rearing.
8. No asexually reproducing populations of T. absoluta have been detected in Argentina meaning that SIT remains viable for Argentinian populations.
9. The presence of Wolbachia in T. absoluta from India (Supergroup A and B) and Argentina (Supergroup B) means that cytoplasmic incompatibility is a possible tool for T. absoluta management.
10. Biocontrol agents like Chrysoperla sp., Tupiocoris sp. Trichogramma sp., have been validated as viable control agents for T. absoluta and S. littoralis.
The results achieved during this CRP have now made it possible to manage Drosophila suzukii and Tuta absoluta with the sterile insect technique and with biological control agents. Key diet development and dose response steps have been achieved to realize the implementation of the sterile insect technique and inherited sterility technique for future Helicoverpa armigera and Spodoptera littoralis management.
Relevance
The CRP pioneered the development of the sterile insect technique for Drosophila suzukii and Tuta absoluta. The results of the research undertaken during the CRP have provided novel information regarding the dose response, mass rearing diet and insect collection and the use of biological control agents for population suppression of Drosophila suzukii and Tuta absoluta. Furthermore, the methodologies have been transferred to Helicoverpa armigera and Spodoptera littoralis. Specific mass rearing and irradiation recommendations have been provided in an IAEA guideline document. Semi-field trials have validated the SIT and BCA strategies as viable options for D. suzukii and T. absoluta. Wolbachia was identified from Tuta populations, paving the way for the development of management tools utilizing cytoplasmic incompatibility.
CRP Publications
Type
Reserch article
Year
2018
Publication URL
https://onlinelibrary.wiley.com/doi/10.1111/jen.12469
Description
Environmentally sustainable pest control options for Drosophila suzukii
Country/Organization
Germany Institute for Insect Biotechnology, Justus-Liebig-University Giessen, Giessen, Germany
Type
Reserch article
Year
2019
Publication URL
https://doi.org/10.1371/journal.pone.0226582
Country/Organization
Austria / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Type
Reserch article
Year
2017
Publication URL
https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0180821&t…
Description
The sterile insect technique for the management of the spotted wing drosophila, Drosophila suzukii: Establishing the optimum irradiation dose
Country/Organization
Canada / Austria - Institut de recherche et de de´veloppement en agroenvironnement, Saint-Bruno-de-Montarville, Que´bec, - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Type
Reserch article
Year
2019
Publication URL
https://link.springer.com/content/pdf/10.1007/s10526-018-09914-0.pdf
Description
Augmentative releases of Trichopria drosophilae for the suppression of early season Drosophila suzukii populations
Country/Organization
USA / Italy - Department of Horticulture, Oregon State University / Technology Transfer Centre, Fondazione Edmund Mach, Italy
Type
Research article
Year
2021
Description
Effect of low cost diet on the cotton leaf worm , Spodoptera littoralis nucleopolyhedrosis virus pathogenicity and sterile insect technique
Country/Organization
Biological Application Department, Nuclear research center, Atomic Energy Authority, Cairo, Egypt , Department of plant protection Faculty of Agriculture, Fayoum University joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Type
Reserch article
Year
2019
Publication URL
https://link.springer.com/content/pdf/10.1007/s10340-017-0944-y.pdf
Description
Sterile insect technique and Wolbachia symbiosis as potential tools for the control of the invasive species Drosophila suzukii
Country/Organization
Austria - France / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture - Université de Rennes
Type
Reserch article
Year
2019
Publication URL
https://link.springer.com/content/pdf/10.1007/s10526-018-09914-0.pdf
Description
Augmentative releases of Trichopria drosophilae for the suppression of early season Drosophila suzukii populations
Country/Organization
USA / Italy - Department of Horticulture, Oregon State University / Technology Transfer Centre, Fondazione Edmund Mach, Italy
Type
Institute for Insect Biotechnology, Justus-Liebig-University Giessen, Giessen, Germany
Year
2018
Publication URL
https://onlinelibrary.wiley.com/doi/10.1111/jen.12469
Description
Environmentally sustainable pest control options for Drosophila suzukii
Country/Organization
Germany
Type
Research article
Year
2017
Publication URL
https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0180821&t…
Description
The sterile insect technique for the management of the spotted wing drosophila, Drosophila suzukii: Establishing the optimum irradiation dose
Country/Organization
Canada / Austria - Institut de recherche et de de´veloppement en agroenvironnement, Saint-Bruno-de-Montarville, Que´bec, - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Type
Reserch article
Year
2020
Publication URL
https://academic.oup.com/jee/advance-article/doi/10.1093/jee/toaa022/5738996
Description
Mass Rearing, Quality Parameters, and Bioconversion in Drosophila suzukii (Diptera: Drosophilidae) for Sterile Insect Technique Purposes
Country/Organization
Mexico / Programa Moscafrut SADER-SENASICA, Subdirección de Desarrollo de Métodos, Camino a Cacaotales s/n 30860, Metapa de Domínguez, Chiapas, México
Type
Research article
Year
2018
Publication URL
https://www.informaticsjournals.com/index.php/jbc/article/view/23115
Description
Molecular characterisation of tomato leaf miner Tuta absoluta populations obtained from different geographical locations of India.
Country/Organization
India- Nuclear Agriculture & Biotechnology Division, Bhabha Atomic Research Centre
Type
Institute for Insect Biotechnology, Justus-Liebig-University Giessen, Giessen, Germany
Year
2018
Publication URL
https://onlinelibrary.wiley.com/doi/10.1111/jen.12469
Description
Environmentally sustainable pest control options for Drosophila suzukii
Country/Organization
Germany
Type
Research article
Year
2018
Publication URL
https://onlinelibrary.wiley.com/doi/10.1111/jen.12469
Description
Environmentally sustainable pest control options for Drosophila suzukii
Country/Organization
Germany Institute for Insect Biotechnology, Justus-Liebig-University Giessen, Giessen, Germany
Type
Reserch article
Year
2020
Publication URL
https://academic.oup.com/jee/advance-article/doi/10.1093/jee/toaa022/5738996
Description
Mass Rearing, Quality Parameters, and Bioconversion in Drosophila suzukii (Diptera: Drosophilidae) for Sterile Insect Technique Purposes
Country/Organization
Mexico / Programa Moscafrut SADER-SENASICA, Subdirección de Desarrollo de Métodos, Camino a Cacaotales s/n 30860, Metapa de Domínguez, Chiapas, México
Type
Reserch article
Year
2019
Publication URL
https://link.springer.com/content/pdf/10.1007/s10526-018-09914-0.pdf
Description
Augmentative releases of Trichopria drosophilae for the suppression of early season Drosophila suzukii populations
Country/Organization
USA / Italy - Department of Horticulture, Oregon State University / Technology Transfer Centre, Fondazione Edmund Mach, Italy
Type
Research article
Year
2020
Publication URL
https://ejbpc.springeropen.com/articles/10.1186/s41938-019-0201-1
Description
Increasing the efficacy of the cotton leaf worm Spodoptera littoralis nucleopolyhedrosis virus using certain essential oils
Country/Organization
Biological Application Department, Nuclear research center, Atomic Energy Authority, Cairo, Egypt joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Type
Research article
Year
2019
Publication URL
https://link.springer.com/content/pdf/10.1007/s10526-018-09914-0.pdf
Description
Augmentative releases of Trichopria drosophilae for the suppression of early season Drosophila suzukii populations
Country/Organization
USA / Italy - Department of Horticulture, Oregon State University / Technology Transfer Centre, Fondazione Edmund Mach, Italy
Type
Reserch article
Year
2022
Description
Polyandry is an important reproductive trait of the invasive Drosophila suzukii
Country/Organization
Italy, University of Pavia, Department of Biology and Biotechnology
Type
Scientific poster
Year
2022
Publication URL
https://www.11isffei.com/program
Country/Organization
Austria, Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Type
Reserch article
Year
2019
Publication URL
https://doi.org/10.3390/insects10120448
Description
Mass-Rearing of Drosophila suzukii for Sterile Insect Technique Application: Evaluation of Two Oviposition Systems
Country/Organization
Austria / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Type
Research article
Year
2021
Publication URL
https://doi.org/10.1111/jen.12894
Description
Hypoxia combined with chilling maintains the quality of irradiated Drosophila flies: a simulated shipment experiment
Country/Organization
Austria / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Type
_Research article
Year
2021
Publication URL
https://aj.tubitak.gov.tr/agriculture/issues/tar-21-45-1/tar-45-1-10-2006-12.pdf
Description
Analysis of parthenogenesis and molecular detection of reproductive symbionts in Tuta absoluta (Lepidoptera: Gelechiidae) from Argentina. Entomologia Experimentalis et applicata.
Country/Organization
Argentina - Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina.
Type
Reserch article
Year
2019
Publication URL
https://doi.org/10.1371/journal.pone.0226582
Description
Irradiation dose response under hypoxia for the application of the sterile insect technique in Drosophila suzukii
Country/Organization
Austria / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Type
Reserch article
Year
2020
Publication URL
https://academic.oup.com/jee/advance-article/doi/10.1093/jee/toaa022/5738996
Description
Mass Rearing, Quality Parameters, and Bioconversion in Drosophila suzukii (Diptera: Drosophilidae) for Sterile Insect Technique Purposes
Country/Organization
Mexico / Programa Moscafrut SADER-SENASICA, Subdirección de Desarrollo de Métodos, Camino a Cacaotales s/n 30860, Metapa de Domínguez, Chiapas, México
Type
Research article
Year
2018
Publication URL
https://doi.org/10.1093/jee/tox349
Description
Effects of irradiation dose on sterility induction and quality parameters of Drosophila suzukii (Diptera: Drosophilidae)
Country/Organization
Brazil, Departamento de Fitossanidade, Universidade Federal de Pelotas, Pelotas, RS, Brazil. Type: Research article
Type
Research article
Year
2018
Description
Effect of gamma irradiation on the susceptibility of the cotton leaf worm, Spodoptera littoralis (Bosid.) (Lepidoptera: Noctuidae) to the infection with nucleopolyhedrosis virus
Country/Organization
Biological Application Department, Nuclear research center, Atomic Energy Authority, Cairo, Egypt and Faculty of Agriculture, Department of Economic Entomology and pesticide, Cairo university Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Type
Reserch article
Year
2018
Publication URL
https://link.springer.com/content/pdf/10.1007/s10340-017-0944-y.pdf
Description
Sterile insect technique and Wolbachia symbiosis as potential tools for the control of the invasive species Drosophila suzukii
Country/Organization
Austria - France / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture - Université de Rennes
Type
Reserch article
Year
2019
Publication URL
https://doi.org/10.3390/insects10120448
Description
Mass-Rearing of Drosophila suzukii for Sterile Insect Technique Application: Evaluation of Two Oviposition Systems
Country/Organization
Austria / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Type
Scientific presentation
Year
2022
Description
Unravelling the reproductive biology traits of Drosophila suzukii as a basis for specific and long-term control of this species
Country/Organization
Italy, University of Pavia, Department of Biology and Biotechnology
Type
Reserch article
Year
2019
Publication URL
https://doi.org/10.3390/insects10120448
Description
Mass-Rearing of Drosophila suzukii for Sterile Insect Technique Application: Evaluation of Two Oviposition Systems
Country/Organization
Austria / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Type
Reserch article
Year
2017
Publication URL
https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0180821&t…
Description
The sterile insect technique for the management of the spotted wing drosophila, Drosophila suzukii: Establishing the optimum irradiation dose
Country/Organization
Canada / Austria - Institut de recherche et de de´veloppement en agroenvironnement, Saint-Bruno-de-Montarville, Que´bec, - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Type
Research article
Year
2018
Publication URL
https://bulletin.incas.ro/
Description
Development of ecofriendly sterile insect technique for agriculturally important insect pests
Country/Organization
India- Nuclear Agriculture & Biotechnology Division, Bhabha Atomic Research Centre
Type
Research article
Year
2019
Publication URL
https://doi.org/10.1371/journal.pone.0226582
Description
Mass-Rearing of Drosophila suzukii for Sterile Insect Technique Application: Evaluation of Two Oviposition Systems
Country/Organization
Austria / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Type
Research article
Year
2019
Publication URL
https://doi.org/10.1111/jen.12563
Description
Radiation effects on Drosophila suzukii (Diptera: Drosophilidae) reproductive behaviour
Country/Organization
Brazil, Departamento de Fitossanidade, Universidade Federal de Pelotas, Pelotas, RS, Brazil. Type: Research article
Type
Reserch article
Year
2019
Publication URL
https://doi.org/10.1371/journal.pone.0226582
Country/Organization
Austria / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Type
Reserch article
Year
2017
Publication URL
https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0180821&t…
Description
The sterile insect technique for the management of the spotted wing drosophila, Drosophila suzukii: Establishing the optimum irradiation dose
Country/Organization
Canada / Austria - Institut de recherche et de de´veloppement en agroenvironnement, Saint-Bruno-de-Montarville, Que´bec, - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Type
Research article
Year
2019
Publication URL
https://doi.org/10.3958/059.044.0307
Description
Effect of irradiation on quality and fertility parameters of Drosophila suzukii in Mexico
Country/Organization
México, SENASICA-CNRF, Carretera Federal México-Pachuca Km 37.5 C.P. 55740, Tecámac, Estado de México Departamento de Fitossanidade
Type
Research article
Year
2021
Publication URL
https://doi.org/10.1038/s41598-021-90034-8
Description
The pest kill rate of thirteen natural enemies as aggregate evaluation criterion of their biological control potential of Tuta absoluta. Scientific Reports. 11, 10756.
Country/Organization
Argentina - Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina.
Type
Reserch article
Year
2018
Publication URL
https://link.springer.com/content/pdf/10.1007/s10340-017-0944-y.pdf
Description
Sterile insect technique and Wolbachia symbiosis as potential tools for the control of the invasive species Drosophila suzukii
Country/Organization
Austria - France / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture - Université de Rennes
Type
Research article
Year
2021
Description
Pathogenicity and virulence of Isaria javanica, Metarhizium anisopliae, and Beauveria bassiana strains for control of Drosophila suzukii (Matsumura)
Country/Organization
Organization: Mexico / Programa Moscafrut SADER-SENASICA, Subdirección de Desarrollo de Métodos, Camino a Cacaotales s/n 30860, Metapa de Domínguez, Chiapas, México
