IAEA Reference Materials
Reference
Sheets
IAEA-A-13
TRACE ELEMENTS IN FREEZE DRIED ANIMAL BLOOD
Intended Use
This
sample is intended to be used as a reference material for the measurement
of trace elements in blood. It can also be used as a quality control material
for the assessment of a laboratory's analytical work, for the validation of
analytical methods and for quality assurance within a laboratory.
Origin
and preparation of the material
Fresh
bovine blood was obtained from a slaughter house and was freeze dried at Seibersdorf.
A batch of 40 kg of the dried material was prepared and further processed
in a ball-mill. The fraction that passed through a 300 µm nylon sieve was
further homogenized by mixing in a rotating plastic drum for 70 hours. The
material was dispensed into plastic bottles in 25 g units without any further
processing. Subsequently, the samples were irradiated to a dose of 2.5 x 104
Gy using a 60Co source to ensure long-term stability of the
material by inhibiting microbial action.
Date of Release:
March 1983
|
Unit Price:
90 €
|
Unit Size:
25 g
|
| Producer:
Chemistry Unit Agency's Laboratories, Seibersdorf A-2444 Seibersdorf, Austria |
Supplier:
Analytical Quality Control Services Agency's Laboratories, Seibersdorf A-2444 Seibersdorf, Austria |
|
Reference Sheet:  IAEA-A-13(.pdf) |
||
| |
|
|
|
|
Br |
R |
22 |
19 – 24 |
13 |
Ca |
R |
286 |
226 – 332 |
18 |
Cu |
R |
4.3 |
3.7 – 4.8 |
32 |
Fe |
R |
2400 |
2200 – 2500 |
34 |
K |
R |
2500 |
2100 – 2700 |
18 |
Mg |
I |
99 |
81 – 139 |
16 |
Na |
R |
12600 |
11600 – 13500 |
19 |
Ni |
I |
1.0 |
0.6 – 1.4 |
4 |
P |
I |
940 |
690 – 1120 |
5 |
Pb |
I |
0.18 |
0.15 – 0.29 |
5 |
Rb |
R |
2.3 |
1.7 – 3.1 |
10 |
S |
R |
6500 |
6000 – 7000 |
4 |
Se |
R |
0.24 |
0.15 – 0.31 |
7 |
Zn |
R |
13 |
12 – 14 |
34 |
| * |
Number of accepted laboratory results which were used to calculate the
recommended or information values and their respective confidence intervals
about the median value. |
| (R/I) | Classification
assigned to the property value for Analyte (Recommended/Information) |
The values listed
above were established on the basis of statistically valid results submitted
by laboratories which had participated in an international intercomparison
exercise organized during 1981. The details concerning the criteria for qualification
as a recommended value can be found in the report (IAEA/RL/98) "Report
on the Intercomparison Run A-13: Trace Elements in Freeze Dried Animal Blood"
[1]. This report is available free of charge upon request.
Homogeneity
The
between and within bottle homogeneity of the material was assessed by determining
the concentrations of potassium and zinc in 200 mg sub-samples using neutron
activation analysis (NAA). The results were subjected to F and T tests and
it was concluded that the material could be considered to be homogeneous for
a sample size greater than or equal to 200 mg.
Dry
weight determination
All
recommended values are expressed on a dry weight basis. Therefore the dry
weight must be determined at the time of analysis, using separate sub-samples
of at least 500 mg dried to constant weight in a drying oven set to 105 °C.
Subsequent weighings should differ by less than 5 mg.
Instructions
for use
The
recommended sample size for analysis is 200 mg. Analysts are reminded to take
appropriate precautions in order to avoid contamination of the material during
handling. No special precautions are required for the storage of this material.
References:
| [1] |
Last updated 30 March 2004
