IAEA Reference Materials

Reference Sheets

REFERENCE MATERIAL
IAEA-432
ORGANOCHLORINE COMPOUNDS AND PETROLEUM HYDROCARBONS
IN
MUSSEL HOMOGENATE

Intended Use  
    This sample is intended to be used as a reference material for the determination of chlorinated compounds and petroleum hydrocarbons in biota samples. It can also be used as a quality control material for the assessment of a laboratory's analytical work, for the validation of analytical methods and for quality assurance within a laboratory.   

Origin and preparation of the material  
    A large batch of mussels (Mytilus edulis) was collected from the North Sea to be used as an intercomparison material. This material was freeze-dried and ground. It was further sieved through a 250 µm stainless steel sieve.
    The mussel powder fraction of particle size less than 250 µm was further homogenized by mixing in a rotating drum for two weeks. Then, aliquots of about 45 grams were packaged into glass bottles with aluminum screw caps and sealed with Teflon tape.
Date of Release:  
May 2004
 Unit Price:  
90 €
 Unit Size:  
40 g
Producer:  
Marine Environment Laboratory (MEL)
International Atomic Energy Agency 
4 Quai Antoine 1er 
MC-98000 Monaco		 Supplier:  
Analytical Quality Control Services 
Agency's Laboratories, Seibersdorf 
A-2444 Seibersdorf, Austria
Reference Sheet:  IAEA-432(.pdf)

Analyte
Status
Concentration
[ng/g]
(Based on dry weight)
Standard deviation
[ng/g]
N *
Chlorinated pesticides
alpha- HCH
I
0.23
0.25
8
beta- HCH
I
0.22
0.11
7
gamma- HCH (Lindane)
I
0.58
0.54
30
HCB
R
0.2
0.1
21
pp’ DDE
R
2.1
1.0
39
pp’ DDD
R
0.88
0.49
30
pp’ DDT
I
0.67
0.46
25
op DDE
I
0.62
0.64
5
op DDD
I
1.8
2.7
4
op DDT
I
1.8
2.0
4
Aldrin
I
1.5
1.4
17
Endrin
I
5.4
6.9
16
Dieldrin
I
1.3
0.94
24
Heptachlor
I
1.0
1.0
19
Heptachlor Epoxide
I
11
18
12
alpha- Endosulfan
I
4.0
3.6
11
beta- Endosulfan
I
1.8
2.0
11
Endosulfan Sulfate
I
1.8
1.3
8
alpha- Chlordane
I
0.25
0.1
6
gamma- Chlordane
I
0.49
0.58
8
Cis Nonachlor
I
0.2
0.17
3
Trans Nonachlor
I
0.53
0.29
3
Aroclor 1254
I
27
17
16
Aroclor 1260
I
16
12
15
Hydrocarbons
Total Aliphatics
I
10000
9000
5
Unresolved Aliphatics
I
38000
43000
5
n - C 17
I
200
140
12
Pristane
I
140
130
10
n - C 18
I
67
68
11
Phytane
I
41
44
9
SUM n Alkanes (C 14 - C 34)
I
4500
4800
10
Phenanthrene
R
27
21
32
1 Methyl Phenanthrene
R
4.2
2.8
8
2 Methyl Phenanthrene
R
9.4
4.9
5
Anthracene
R
1.5
1.1
11
Chrysene
R
5.5
3.8
23
Fluoranthene
R
12
6.5
27
Fluorene
R
4.1
2.2
8
Pyrene
R
13
6.0
25
Benzo (b) Fluoranthene
R
4.8
1.7
10
Benzo (k) Fluoranthene
R
1.9
1.1
12
Benz (a) Anthracene
R
3.8
3.1
24
Benzo (a) Pyrene
R
0.9
0.5
17
Benzo (e) Pyrene
R
4.5
1.7
11
Naphthalene
I
15
18
15
Perylene
I
5.0
2.8
3
UVF equival. ROPME oil
I
11000
4900
3
Resolved Aliphatics
I
18000
20000
9
Total Aromatics
I
3000
3900
8
Resolved Aromatics
I
1500
1800
10
1 Methyl Naphthalene
I
8.8
9.5
3
2 Methyl Naphthalene
I
14
13
3
PCBs
PCB No 8
I
0.57
0.32
4
PCB No 18
I
1.6
1.7
11
PCB No 28
I
0.32
0.26
18
PCB No 31
I
1.0
1.04
10
PCB No 44
I
0.7
0.68
10
PCB No 49
R
0.29
0.08
6
PCB No 52
I
1.2
1.2
23
PCB No 66
I
0.38
0.16
4
PCB No 70
R
0.64
0.35
7
PCB No 74
I
0.13
0.03
4
PCB No 82
I
0.11
0.08
3
PCB No 87
I
0.27
0.11
4
PCB No 95
I
1.1
0.79
8
PCB No 97
I
0.31
0.16
4
PCB No 99
I
2.1
2.4
7
PCB No 101
R
1.2
0.49
24
PCB No 105
I
0.5
0.39
14
PCB No 110
R
1.12
0.4
10
PCB No 118
R
1.09
0.42
24
PCB No 128
I
0.25
0.08
7
PCB No 138
R
2.2
0.84
31
PCB No 141
I
0.19
0.22
3
PCB No 146
I
0.59
0.11
3
PCB No 149
R
1.4
0.41
10
PCB No 151
I
0.4
0.04
3
PCB No 153
R
2.8
0.99
31
PCB No 156
I
0.35
0.31
8
PCB No 158
I
0.17
0.07
4
PCB No 167
I
0.32
0.1
3
PCB No 170
I
0.57
0.51
7
PCB No 174
I
0.26
0.27
4
PCB No 177
I
0.26
0.14
4
PCB No 180
R
0.2
0.11
16
PCB No 183
I
0.24
0.05
6
PCB No 187
I
0.87
0.13
7
PCB No 189
I
0.12
0.15
3
PCB No 194
I
0.27
0.28
4
PCB No 195
I
0.22
0.18
5

*
Number of accepted laboratory results which were used to calculate the recommended or information values and their respective confidence intervals about the mean value.
(R/I)  Classification assigned to the property value for Analyte (Recommended/Information)


The values listed above were established on the basis of statistically valid results submitted by laboratories which had participated in an international intercomparison exercise conducted in 2003. The details concerning the criteria for qualification as a recommended or information value can be found in the report (IAEA/AL/146; IAEA/MEL/74), "World-wide and Regional Intercomparison for Determination of Organochlorine Compounds and Petroleum Hydrocarbons in Mussel Tissue IAEA-432" [1]. This report is available free of charge upon request.    

Homogeneity  
    The homogeneity of the material for organochlorine compounds and petroleum hydrocarbons was checked by determining the concentration of some compounds (chlorinated pesticides and petroleum hydrocarbons) in 10 replicate analyses taken randomly in the bulk of the powder. A one-way variance analysis indicated that the material could be considered homogeneous.

Dry weight determination  
    The moisture content of the lyophilized sample as determined by drying to a constant weight at 105°C, was found to be 2.5 %. Since the moisture content can change with the ambient humidity and temperature, it is recommended that the water content of this material always be determined in a separate sub-sample (not that taken for analysis) by drying to a constant weight (~24 hours) at 105°C. Results should always be reported on a dry weight basis.  

Instructions for use  
    The recommended sample size for analysis is 2 g for petroleum hydrocarbons and 5 g for organochlorine pesticides and PCBs. Analyst are reminded to take appropriate precautions in order to avoid contamination of the material during handling. The material should be stored in the dark and kept in a refrigerator.  

References: 
[1]

Last updated 5 May 2004


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