Links

IAEA Reference Materials

Reference Sheets

REFERENCE MATERIAL
IAEA-406
ORGANOCHLORINE COMPOUNDS AND PETROLEUM HYDROCARBONS
IN
FISH HOMOGENATE

Intended Use
This sample is intended to be used as a reference material for the determination of chlorinated compounds and petroleum hydrocarbons in fish samples. It can also be used as a quality control material for the assessment of a laboratory's analytical work, for the validation of analytical methods and for quality assurance within a laboratory.

Origin and preparation of the material
    A large batch of fish powder was received from an aquaculture farm for use as an intercomparison material. This material was received already freeze-dried and ground. It was further sieved through a 250 µm stainless steel sieve.
The fish powder fraction of particle size less than 250 µm was further homogenized by mixing in a rotating drum for two weeks. Then, aliquots of about 35 grams were packaged into glass bottles with aluminium screw caps and sealed with Teflon tape.

Date of Release:

November 2000
Unit Price:
90 €
Unit Size:
35 g

Producer:
Marine Environment Laboratory (MEL)
International Atomic Energy Agency
B.P. No 800
MC-98012 Monaco Supplier:
Analytical Quality Control Services
Agency's Laboratories, Seibersdorf
A-2444 Seibersdorf, Austria

Reference Sheet: IAEA-406(.pdf)

Analyte Status Concentration
[ng/g] (Based on dry weight) 95% C. I.
[ng/g] N *

Chlorinated pesticides

alpha- HCH I 0.79 0.23 - 1.7 10

beta- HCH I 0.75 0.20 - 2.3 6

gamma- HCH (Lindane) I 0.27 0.11 - 0.80 23

HCB R 1.5 0.95 - 2.0 28

pp’ DDE R 9.2 6.2 - 11 37

pp’ DDD R 2.8 2.0 - 3.7 32

pp’ DDT I 3.0 1.8 - 5.6 37

op DDE R 0.76 0.48 - 1.3 4

op DDD I 0.88 0.43 - 3.0 3

op DDT I 2.9 0.90 - 4.4 6

Aldrin R 0.75 0.61 - 1.2 12

Endrin I 1.9 0.86 - 5.1 16

Dieldrin I 3.5 1.4 - 7.0 23

Heptachlor R 0.32 0.23 - 0.46 10

Heptachlor Epoxide I 0.99 0.37 - 1.6 14

alpha- Endosulfan I 3.5 0.94 - 4.7 12

beta- Endosulfan I 1.4 1.0 - 1.6 7

Endosulfan Sulfate I 3.6 3.1 - 5.6 6

alpha- Chlordane R 2.8 2.0 - 4.1 13

gamma- Chlordane I 0.70 0.63 - 1.0 7

Cis Nonachlor I 0.86 0.77 - 1.4 3

Trans Nonachlor I 4.1 3.9 - 4.1 3

Aroclor 1254 I 33 26 - 58 11

Aroclor 1260 I 13 11 - 15 6

Hydrocarbons

Total Aliphatics I 33000 28000 - 34000 3

n - C 17 I 91 30 - 260 8

Pristane I 3500 720 - 6200 11

n - C 18 I 240 38 - 560 10

Phytane I 200 13 - 310 6

SUM n Alkanes (C 14 - C 34) I 4500 2800 - 22000 8

Phenanthrene I 22 8 - 220 12

1 Methyl Phenanthrene I 12 3.6 - 92 4

2 Methyl Phenanthrene I 4.7 3.3 - 10 3

Anthracene I 19 15 - 43 4

Chrysene I 2.3 2.2 - 4.4 5

Fluoranthene I 4.9 4.0 - 9.6 9

Pyrene R 4.5 2.1 - 9.1 7

Benzo (b) Fluoranthene I 2.3 0.78 - 9.5 4

Benzo (k) Fluoranthene I 2.8 0.38 - 130 4

Benz (a) Anthracene I 0.81 0.24 - 0.91 5

Benzo (a) Pyrene I 2.9 0.62 - 3.0 5

Naphthalene I 24 16 - 440 9

Benzo (ghi) Perylene I 0.78 0.28 - 1.3 4

UVF equival. ROPME oil I 8400 3500 - 16000 4

Resolved Aliphatics I 24000 8800 - 310000 4

Total Aromatics I 26000 7200 - 53000 5

Resolved Aromatics I 35000 8100 - 55000 4

Acenaphthene I 1100 0.29 - 2300 4

Acenaphthylene I 8.0 1.1 - 26 3

PCBs

PCB No 18 I 0.38 0.11 - 0.93 5

PCB No 28 R 0.57 0.43 - 1.3 18

PCB No 31 R 0.29 0.23 - 0.47 5

PCB No 44 I 0.46 0.46 - 0.52 5

PCB No 45 I 0.18 0.03 - 0.33 3

PCB No 49 I 0.41 0.36 - 0.44 4

PCB No 52 R 1.3 1.0 - 2.2 15

PCB No 66 I 0.67 0.49 - 0.96 4

PCB No 87 I 0.82 0.69 - 1.4 4

PCB No 95 I 1.1 1.0 - 1.2 3

PCB No 99 I 0.91 0.89 - 1.2 3

PCB No 101 R 3.1 2.2 - 3.4 18

PCB No 105 I 0.71 0.48 - 0.88 9

PCB No 110 R 1.4 1.38 - 2.0 5

PCB No 118 I 2.5 1.9 - 3.7 20

PCB No 128 I 0.8 0.51 - 1.4 5

PCB No 138 R 4.0 2.5 - 6.3 23

PCB No 141 I 0.41 0.40 - 0.43 4

PCB No 149 R 2.0 1.7 - 2.4 6

PCB No 151 I 0.67 0.62 - 0.75 4

PCB No 153 R 3.7 2.9 - 6.0 22

PCB No 156 R 0.27 0.21 - 0.59 6

PCB No 170 R 0.54 0.38 - 0.81 7

PCB No 174 I 0.30 0.29 - 0.37 3

PCB No 177 I 0.18 0.16 - 0.19 4

PCB No 180 R 1.2 1.0 - 1.2 14

PCB No 183 I 0.32 0.28 - 0.35 4

PCB No 187 I 1.1 0.96 - 1.2 6

PCB No 194 I 0.13 0.10 - 0.25 4

PCB No 195 I 0.04 0.03 - 1.5 3

PCB No 198 I 0.068 0.009 - 0.500 3

PCB No 199 I 0.16 0.013 - 0.17 3

PCB No 200 I 0.10 0.009 - 1.1 3

PCB No 201 I 0.12 0.07 - 0.23 3

PCB No 206 I 0.05 0.03 - 3.1 3

PCB No 209 I 0.07 0.07 - 1.1 4

*
Number of accepted laboratory results which were used to calculate the recommended or information values and their respective confidence intervals about the median value.

(R/I) Classification assigned to the property value for Analyte (Recommended/Information)

The values listed above were established on the basis of statistically valid results submitted by laboratories which had participated in an international intercomparison exercise organized in 2000. The details concerning the criteria for qualification as a recommended or information value can be found in the report (IAEA/AL/125; IAEA/MEL/69), “World-wide and Regional Intercomparison for Determination of Organochlorine Compounds and Petroleum Hydrocarbons in Fish Homogenate IAEA-406” [1]. This report is available free of charge upon request.

Homogeneity
    The homogeneity of the material for organochlorine compounds and petroleum hydrocarbons was checked by determining the concentration of some compounds (chlorinated pesticides and petroleum hydrocarbons) in 10 replicate analyses taken randomly in the bulk of the powder. A one-way variance analysis indicated that the material could be considered homogeneous.

Dry weight determination
    The moisture content of the lyophilized sample as determined by drying to a constant weight at 105°C, was found to be 11 %. Since the moisture content can change with the ambient humidity and temperature, it is recommended that the water content of this material always be determined in a separate sub-sample (not that taken for analysis) by drying to a constant weight (~24 hours) at 105°C. Results should always be reported on a dry weight basis.

Instructions for use
The recommended sample size for analysis is 2 g for petroleum hydrocarbons and 5 g for organochlorine pesticides and PCBs. Analyst are reminded to take appropriate precautions in order to avoid contamination of the material during handling. The material should be stored in the dark and kept in a refrigerator.

References:

[1]
Villeneuve J-P., de Mora S. J. and Cattini C., World-wide and Regional Intercomparison for Determination of Organochlorine Compounds and Petroleum Hydrocarbons in Fish Homogenate IAEA-406; IAEA/AL/125 (IAEA/MEL/69), IAEA, Monaco, 2000.

Last updated 30 March 2004

*Date of Release:* November 2000	*Unit Price:* 90 €	*Unit Size:* 35 g
*Producer:* Marine Environment Laboratory (MEL) International Atomic Energy Agency B.P. No 800 MC-98012 Monaco	*Supplier:* Analytical Quality Control Services Agency's Laboratories, Seibersdorf A-2444 Seibersdorf, Austria
Reference Sheet: IAEA-406(.pdf)

Analyte	Status	Concentration [ng/g] *(Based on dry weight)*	95% C. I. [ng/g]	N *
Chlorinated pesticides
alpha- HCH	I	0.79	0.23 - 1.7	10
beta- HCH	I	0.75	0.20 - 2.3	6
gamma- HCH (Lindane)	I	0.27	0.11 - 0.80	23
HCB	R	1.5	0.95 - 2.0	28
pp’ DDE	R	9.2	6.2 - 11	37
pp’ DDD	R	2.8	2.0 - 3.7	32
pp’ DDT	I	3.0	1.8 - 5.6	37
op DDE	R	0.76	0.48 - 1.3	4
op DDD	I	0.88	0.43 - 3.0	3
op DDT	I	2.9	0.90 - 4.4	6
Aldrin	R	0.75	0.61 - 1.2	12
Endrin	I	1.9	0.86 - 5.1	16
Dieldrin	I	3.5	1.4 - 7.0	23
Heptachlor	R	0.32	0.23 - 0.46	10
Heptachlor Epoxide	I	0.99	0.37 - 1.6	14
alpha- Endosulfan	I	3.5	0.94 - 4.7	12
beta- Endosulfan	I	1.4	1.0 - 1.6	7
Endosulfan Sulfate	I	3.6	3.1 - 5.6	6
alpha- Chlordane	R	2.8	2.0 - 4.1	13
gamma- Chlordane	I	0.70	0.63 - 1.0	7
Cis Nonachlor	I	0.86	0.77 - 1.4	3
Trans Nonachlor	I	4.1	3.9 - 4.1	3
Aroclor 1254	I	33	26 - 58	11
Aroclor 1260	I	13	11 - 15	6
Hydrocarbons
Total Aliphatics	I	33000	28000 - 34000	3
n - C 17	I	91	30 - 260	8
Pristane	I	3500	720 - 6200	11
n - C 18	I	240	38 - 560	10
Phytane	I	200	13 - 310	6
SUM n Alkanes (C 14 - C 34)	I	4500	2800 - 22000	8
Phenanthrene	I	22	8 - 220	12
1 Methyl Phenanthrene	I	12	3.6 - 92	4
2 Methyl Phenanthrene	I	4.7	3.3 - 10	3
Anthracene	I	19	15 - 43	4
Chrysene	I	2.3	2.2 - 4.4	5
Fluoranthene	I	4.9	4.0 - 9.6	9
Pyrene	R	4.5	2.1 - 9.1	7
Benzo (b) Fluoranthene	I	2.3	0.78 - 9.5	4
Benzo (k) Fluoranthene	I	2.8	0.38 - 130	4
Benz (a) Anthracene	I	0.81	0.24 - 0.91	5
Benzo (a) Pyrene	I	2.9	0.62 - 3.0	5
Naphthalene	I	24	16 - 440	9
Benzo (ghi) Perylene	I	0.78	0.28 - 1.3	4
UVF equival. ROPME oil	I	8400	3500 - 16000	4
Resolved Aliphatics	I	24000	8800 - 310000	4
Total Aromatics	I	26000	7200 - 53000	5
Resolved Aromatics	I	35000	8100 - 55000	4
Acenaphthene	I	1100	0.29 - 2300	4
Acenaphthylene	I	8.0	1.1 - 26	3
PCBs
PCB No 18	I	0.38	0.11 - 0.93	5
PCB No 28	R	0.57	0.43 - 1.3	18
PCB No 31	R	0.29	0.23 - 0.47	5
PCB No 44	I	0.46	0.46 - 0.52	5
PCB No 45	I	0.18	0.03 - 0.33	3
PCB No 49	I	0.41	0.36 - 0.44	4
PCB No 52	R	1.3	1.0 - 2.2	15
PCB No 66	I	0.67	0.49 - 0.96	4
PCB No 87	I	0.82	0.69 - 1.4	4
PCB No 95	I	1.1	1.0 - 1.2	3
PCB No 99	I	0.91	0.89 - 1.2	3
PCB No 101	R	3.1	2.2 - 3.4	18
PCB No 105	I	0.71	0.48 - 0.88	9
PCB No 110	R	1.4	1.38 - 2.0	5
PCB No 118	I	2.5	1.9 - 3.7	20
PCB No 128	I	0.8	0.51 - 1.4	5
PCB No 138	R	4.0	2.5 - 6.3	23
PCB No 141	I	0.41	0.40 - 0.43	4
PCB No 149	R	2.0	1.7 - 2.4	6
PCB No 151	I	0.67	0.62 - 0.75	4
PCB No 153	R	3.7	2.9 - 6.0	22
PCB No 156	R	0.27	0.21 - 0.59	6
PCB No 170	R	0.54	0.38 - 0.81	7
PCB No 174	I	0.30	0.29 - 0.37	3
PCB No 177	I	0.18	0.16 - 0.19	4
PCB No 180	R	1.2	1.0 - 1.2	14
PCB No 183	I	0.32	0.28 - 0.35	4
PCB No 187	I	1.1	0.96 - 1.2	6
PCB No 194	I	0.13	0.10 - 0.25	4
PCB No 195	I	0.04	0.03 - 1.5	3
PCB No 198	I	0.068	0.009 - 0.500	3
PCB No 199	I	0.16	0.013 - 0.17	3
PCB No 200	I	0.10	0.009 - 1.1	3
PCB No 201	I	0.12	0.07 - 0.23	3
PCB No 206	I	0.05	0.03 - 3.1	3
PCB No 209	I	0.07	0.07 - 1.1	4

*	Number of accepted laboratory results which were used to calculate the recommended or information values and their respective confidence intervals about the median value.
(R/I)	Classification assigned to the property value for Analyte (Recommended/Information)